Inhibitory effect of erigeron breviscapus on inflammation in diabetic nephropathy rats by Psmb8/NF-κB axis
Objective To investigate whether erigeron breviscapus(EB)can mediate proteasome subunit β type 8(Psmb8)/nuclear factor-κB(NF-κB)axis to inhibit inflammation in diabetic nephropathy(DN)rats.Methods Ten SPF male SD rats were randomly selected as the normal group,and the rest rats were treated with streptozotocin(STZ)to construct diabetic nephropathy(DN)rat model.There were 41 model rats,After one rat was randomly eliminated,the remaining rats were randomly divided into model group,EB low-dose group,EB medium-dose group and EB high-dose group,with 10 rats in each group.The EB low,medium and high dose groups were given EB 20,40 and 80 mg·kg-1·d-1,respectively.The normal group and model group were given the same amount of normal saline for 8 weeks.Fasting blood glucose(FBG)and renal mass index(KI)were detected.The levels of 24-hour urine protein(UPro),serum creatinine(Scr)and blood urea nitrogen(BUN)were detected by automatic biochemical analyzer.HE staining was used to observe the pathological changes of renal tissues.The serum levels of tumor necrosis factor α(TNF-α),interleu-kin-1β(IL-1β)and interleukin-18(IL-18)were determined by ELISA.The mRNA relative expression levels of Psmb8 and NF-κB P65 were detected by quantitative real-time polymerase chain reaction(qRT-PCR).The relative protein expression levels of Psmb8,NF-κB p65 and p-NF-κB p65 were detected by Western blotting.Results Compared with normal group,FBG,KI increased,body weight decreased in model group(P＜0.05),the glomerular morphology showed obvious pathological changes,interstitial edema ac-companied by a large numbers of inflammatory cell infiltration,and the serum TNF-α,IL-1β and IL-18 contents increased.The mRNA expression of Psmb8 and NF-κB p65 and the relative protein expression of Psmb8,NF-κB P65 and p-NF-κB p65 were in-creased in renal tissues(P＜0.05).Compared with model group,the FBG and KI were decreased and body weight was increased in low,medium and high dose EB groups(P＜0.05),the inflammatory pathology of renal tissue was alleviated to varying degrees,and the contents of TNF-α,IL-1β and IL-18 in serum were decreased.The mRNA expression of Psmb8 and NF-κB p65 and the relative protein expression of Psmb8,NF-κB p65 and p-NF-κB p65 were decreased in renal tissue(P＜0.05).The effect of EB on DN rats was dose-dependent.Conclusion EB can inhibit inflammatory response in DN rats,and its mechanism may be related to inhibiting the activation of Psmb8/NF-κB axis.
NETL
NSTL
万方数据
维普
