牡丹果荚的质量标准研究
Study on the quality standard of the pods of Paeonia ostii T.Hong & J.X.Zhang
肖会敏 1杨旭 2赵利娟 3李畅 4林奋 2王四旺 4高锦明5
作者信息
- 1. 西北农林科技大学化学与药学院,杨凌 710069;陕西凤丹正元生物科技有限公司研发部,西安 710077
- 2. 陕西凤丹正元生物科技有限公司研发部,西安 710077
- 3. 陕西中药研究所,陕西医药信息中心,咸阳 712000
- 4. 西北大学生命科学与医学部,西安 710069
- 5. 西北农林科技大学化学与药学院,杨凌 710069
- 折叠
摘要
目的 建立牡丹果荚的质量标准.方法 用高效液相色谱法(high performance liquid chromatography,HPLC)测定牡丹果荚中没食子酸、鞣花酸的含量;用薄层色谱法(thin layer chromatography,TLC)鉴别牡丹果荚中没食子酸;按照2020年版《中华人民共和国药典》中的方法测定水分、总灰分、酸不溶性灰分、水溶性浸出物的含量;用生物显微镜观察法对果荚粉末进行鉴别.结果 建立了专属性强、重复性好的药材中没食子酸的TLC鉴别方法;建立了药材中没食子酸及鞣花酸含量测定方法:没食子酸在1.30～166.70µg·mL-1范围内线性关系良好(r=0.999 5),平均加样回收率为96.66％,RSD值为1.95％;鞣花酸在1.00～128.26μg·mL-1范围内线性关系良好(r=0.999 3),平均加样回收率为98.22％,RSD值为1.15％;12批药材中没食子酸、鞣花酸、水分、总灰分、酸不溶性灰分、水溶性浸出物的质量分数分别为7.73～10.00 mg·g-1、11.31～12.50 mg·g-1、9.98％～11.07％、10.35％～12.35％、0.32％～0.41％、32.27％～33.55％;果荚粉末观测到簇晶、导管和非腺毛.结论 所建立的牡丹果荚质量标准可用于该药材的质量控制.
Abstract
Objective To establish the quality standard of the pods of Paeonia ostii T.Hong & J.X.Zhang.Methods High perfor-mance liquid chromatography(HPLC)was used to determine the contents of gallic acid and ellagic acid in the pods of Paeonia ostii T.Hong&J.X.Zhang.Thin layer chromatography(TLC)was used to identify gallic acid.The contents of water,total ash,acid in-soluble ash and water-soluble extract were determined according to Chinese Pharmacopoeia(2020 Edition).The powders of the pods were identified by biological microscope.Results A TLC identification method of gallic acid with strong specificity and good repeat-ability was established.Methods for the determination of gallic acid and ellagic acid was also established.Gallic acid has a good rela-tionship in the linear range of 1.30-166.70 μg·mL-1 with regression coefficient r=0.999 5,and its average recovery was 96.66％with RSD 1.95％.The ellagic acid has a good relationship in the range of 1.00-128.26 μg·mL-1 with regression coefficient r=0.9993,and its average recovery was 98.22％with RSD 1.15％.The mass fraction of gallic acid,ellagic acid,water,total ash,acid in-soluble ash and alcohol soluble extract in 14 batches of the pods were 7.73-10.00 mg·g-1,11.31-12.50 mg·g-1,9.98％—11.07％,10.35％—12.35％,0.32％—0.41％,and 32.27％—33.55％,respectively.Tufts,catheters and non-glandular hairs can be observed in the powder.Conclusion The established HPLC method,TLC method and microscopic method can be used for the qua-lity control of the pods of Paeonia ostii T.Hong & J.X.Zhang.
关键词
牡丹/果荚/薄层色谱法/高效液相色谱法/质量标准/没食子酸/鞣花酸Key words
Paeonia ostii T.Hong & J.X.Zhang/pod/thin layer chromatography/high performance liquid chromatography/qual-ity standard/gallic acid/ellagic acid引用本文复制引用
基金项目
陕西省生物医药重点实验室后补助项目(2018SZS41)
陕西省重点研发计划(2018SF-315)
出版年
2024
NETL
NSTL
万方数据