Study on the quality standard of the pods of Paeonia ostii T.Hong & J.X.Zhang
Objective To establish the quality standard of the pods of Paeonia ostii T.Hong & J.X.Zhang.Methods High perfor-mance liquid chromatography(HPLC)was used to determine the contents of gallic acid and ellagic acid in the pods of Paeonia ostii T.Hong&J.X.Zhang.Thin layer chromatography(TLC)was used to identify gallic acid.The contents of water,total ash,acid in-soluble ash and water-soluble extract were determined according to Chinese Pharmacopoeia(2020 Edition).The powders of the pods were identified by biological microscope.Results A TLC identification method of gallic acid with strong specificity and good repeat-ability was established.Methods for the determination of gallic acid and ellagic acid was also established.Gallic acid has a good rela-tionship in the linear range of 1.30-166.70 μg·mL-1 with regression coefficient r=0.999 5,and its average recovery was 96.66％with RSD 1.95％.The ellagic acid has a good relationship in the range of 1.00-128.26 μg·mL-1 with regression coefficient r=0.9993,and its average recovery was 98.22％with RSD 1.15％.The mass fraction of gallic acid,ellagic acid,water,total ash,acid in-soluble ash and alcohol soluble extract in 14 batches of the pods were 7.73-10.00 mg·g-1,11.31-12.50 mg·g-1,9.98％—11.07％,10.35％—12.35％,0.32％—0.41％,and 32.27％—33.55％,respectively.Tufts,catheters and non-glandular hairs can be observed in the powder.Conclusion The established HPLC method,TLC method and microscopic method can be used for the qua-lity control of the pods of Paeonia ostii T.Hong & J.X.Zhang.
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