Study on the quality standard of Mongolian medicinal herb Eritrichium mandshu-ricum M.Pop.
Objective To establish the quality standard of Eritrichium mandshuricum M.Pop.and provide reference for quality control and evaluation of E.mandshuricum M.Pop..Methods The moisture,total ash,acid-insoluble ash and extracts of E.mandshuricum M.Pop.were determined by referring to the relevant methods compiled in Volume 4,General Chapters in the Chinese Pharmaco-poeia(2020 edition).A method for the quantitative analysis of chlorogenic acid,rutin and isoquercitrin in E.mandshuricum M.Pop.was established by high performance liquid chromatography(HPLC).The separation was performed by gradient elution,using Hy-persil ODS(300 mmX4.6 mm,2.5 μm)as the column at 35 ℃,at a flow rate of 1.0 mL·min-1,with 4 mL·L-1 phosphoric acid-acetonitrile as the mobile phase,with a detection wavelength at 360 nm.Results The moisture content,total ash content and acid in-soluble ash content of E.mandshuricum M.Pop.should not exceed 10.0％,7.0％and 2.0％,respectively,and the extract should not be less than 20％.The mass concentration of chlorogenic acid,rutin and isoquercitrin showed good linear relationship in the range of 1.42～4.25 μg·mL1,0.80～2.40 μg·mL-1and 0.14～0.42 μg·mL-1,respectively.The average recovery rates were 96.33％,95.50％and 95.63％,and RSD were 1.7％,0.9％and 1.4％,respectively.Conclusion The established quantitative method has good repeatability,high sensitivity and specificity,and can be used for the quality control of Mongolian medicinal herb E.mandshuri-cum M.Pop..
Mongolian medicinal herbEritrichium mandshuricum M.Pop.chlorogenic acidrutinisoquercitrinquality standard
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