摘要
目的 探讨茯苓多糖(Poria cocos polysaccharide,PCP)对自身免疫性炎性肌病(autoimmune inflammatory myopathy,IIM)大鼠免疫系统的影响及其可能的作用机制.方法 将造模成功的40只IIM模型大鼠随机分为模型组及PCP低、中、高剂量组,各10只,另设对照组10只.PCP低、中、高剂量组大鼠分别灌胃75、150、300mg·kg-1 PCP溶液(用蒸馏水配制),对照组及模型组给予等量蒸馏水.观察各组大鼠一般情况并记录体质量;观察各组大鼠肌电图变化;用酶联免疫吸附试验(enzyme linked immu-nosorbent assay,ELISA)检测各组大鼠血清肌酸激酶(creatine kinase,CK)、乳酸脱氢酶(lactate dehydrogenase,LDH)和天冬氨酸氨基转移酶(aspartate aminotransferase,AST)水平;称取各组大鼠脾脏、胸腺质量并计算脾脏指数、胸腺指数;用HE染色观察骨骼肌病理学变化;评定大鼠骨骼肌病理损伤情况;用反转录·聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)和蛋白印迹法(Western blotting)分别检测大鼠骨骼肌干扰素调节因子3(interferon regulatory factor 3,IRF-3)、干扰素调节因子7(interferon regulatory factor 7,IRF-7)mRNA和蛋白表达情况.结果 与模型组比较,PCP低剂量组、PCP中剂量组和PCP高剂量组大鼠体质量、运动单位时限、波幅、脾脏指数、胸腺指数、IRF-3与IRF-7 mRNA和蛋白表达水平升高,多项波、CK、LDH、AST和骨骼肌病理评分降低(P＜0.05).与PCP低剂量组比较,PCP中剂量组和PCP高剂量组大鼠体质量、运动单位时限、波幅、脾脏指数、胸腺指数、IRF-3与IRF-7 mRNA和蛋白表达水平升高,多项波、CK、LDH、AST和骨骼肌病理评分均降低(P＜0.05),高剂量组诸项指数水平变化比中剂量组更显著(P＜0.05).结论 PCP能够调节IIM大鼠免疫功能,改善大鼠肌损伤,可能是通过激活IRF-3、IRF-7表达发挥作用的.
Abstract
Objective To investigate the effect of Poria cocos polysaccharide(PCP)on the immune system of autoimmune inflammatory myopathy(IIM)of rats and its possible mechanism.Methods A total of 40 IIM model rats were randomly divided into model group,PCP low-dose,PCP medium-dose and PCP high-dose groups,10 rats in each group,and also a control group(10 rats)was set up.Rats in low,medium and high dosage of PCP groups were administered by gavage with 75,150 and 300 mg·kg-1 PCP solution(prepared with distilled water),and the control group and model group were given the same amount of distilled water.The general conditions of rats in each group were observed and their body mass was recorded.The changes of electromyography were observed.The levels of serum creatine kinase(CK),lactate dehydrogenase(LDH)and aspartate aminotransferase(AST)were detected by enzyme linked immunosorbent assay(ELISA).The weights of spleen and thymus of rats in each group were weighed,and the spleen index and thymus index were calculated.The pathological changes of skeletal muscle were observed by HE staining.The pathological injury of rat skeletal muscle was evaluated.The mRNA and protein expressions of interferon regulatory factor 3(IRF-3)and interferon regulatory factor 7(IRF-7)in rat skeletal muscle were detected by reverse transcription-polymerase chain reaction(RT-PCR)and Western blotting.Results Compared with the model group,the body mass,exercise unit time limit,wave amplitude,spleen index,thymus index,IRF-3 and IRF-7 mRNA and protein expression levels were increased,and the pathological scores of multiple waves,CK,LDH,AST and skeletal muscle of rats in low,medium and high dosage of PCP groups were decreased(P＜0.05).Compared with the low dose of PCP group,the body mass,exercise unit time limit,wave amplitude,spleen index,thymus index,IRF-3 and IRF-7 mRNA and protein expression levels were increased,and the pathological scores of multiple waves,CK,LDH,AST and skeletal muscle of rats in medium and high dose of PCP groups were decreased(P＜0.05).The changes of various indexes in the high dose group were more significant than those in the medium dose group(P＜0.05).Conclusion PCP can regulate immune function and improve muscle injury in IIM rats,which may play a role by activating the expression of IRF-3 and IRF-7.
基金项目
河北省医学科学研究课题(2021)(20211293)
NETL
NSTL
万方数据