Mechanism of airway remodeling effect of the extract of Hyssopus cuspidatus Boriss in asthmatic young rats
Objective To investigate the effect of the extract of Hyssopus cuspidatus Boriss(EHC)on airway remodeling in asthmatic infant rats by regulating epithelial-mesenchymal transition(EMT)through transforming growth factor-β1(TGF-β1)/Mothers against decapentaplegic homologs(Smads)pathway.Methods The young rats were randomly divided into model group,low-dose EHC group(10 mg·kg-1),high-dose EHC group(40 mg·kg-1)and positive drug group(dihydroxypropylline,20 mg·kg-1),and control group,with 20 rats in each group.The number of inflammatory cells and the levels of cytokines interleukin-4(IL-4),matrix metalloproteinase 9(MMP-9)and tumor necrosis factor-α(TNF-α)in bronchoalveolar lavage fluid(BALF)were compared.The lung tissue lesions were observed by HE staining,and the thickness of airway wall and smooth muscle of lung tissue,the mRNA and protein expression of E-cadherin,α-smooth muscle actin(α-SMA),TGF-β1,Mothers against decapentaplegic homolog 2(Smad2),Mothers against decapentaplegic homolog 3(Smad3)were compared.Results Compared with the control group,the number of eosinophil(EOS),neutrophil(NEU)and lymphocyte(LYM)cells,bronchial airway wall and smooth muscle thickness,IL-4,TNF-α and MMP-9 levels,the mRNA and protein levels of α-SMA,TGF-β1 and α-SMA,TGF-β1,p-Smad2 and p-Smad3 were increased,while the mRNA and protein levels of E-cadherin were decreased in model group(P<0.05).Compared with the model group,the number of EOS,NEU and LYM cells,bronchial airway wall and smooth muscle thickness,IL-4,TNF-α and MMP-9 levels,the mRNA and protein levels of E-cadherin increased,while the α-SMA,TGF-β1 mRNA and α-SMA,TGF-β1,p-Smad2,p-Smad3 protein levels decreased in the low and high dose EHC groups and positive drug group(P<0.05);Moreover,the changes in the levels of various indices were the most obvious in the positive drug group,followed by the high dose EHC groups,and the lowest was the low dose EHC groups(P<0.05).HE results showed that,compared with the model group,the injury was improved to different degrees,and the improvement was obvious in the high-dose group and the positive drug group.Conclusion EHC can effectively inhibit airway remodeling in asthmatic young mice,possibly by regulating TGF-β1/Smads pathway to regulate related mRNA and protein expression and inhibit EMT.
extract of Hyssopus cuspidatus Boriss(EHC)asthmaepithelial-mesenchymal transformationairway remodelingtransforming growth factor-β1mothers against decapentaplegic homolog
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NSTL
万方数据
维普
