Objective To establish a gas chromatographic method for the determination of 7 essence components in Acanthopanacis Cortex.Methods The parameters,such as extraction solvent,split ratio and chromatographic column model were examined,and a gas chromatographic method was established by using hydrogen flame ionization detector(FID)and external standard method for quantification.Results When ethyl acetate was used as the extraction solvent and CD-624 capillary column was used for separation,the split ratio of 5∶1 was the best,and the 7 essence components could be completely separated,with good linear relationship,r＞0.999.The average recovery rate of the added standard was 87.33%—93.66%,and the relative standard deviation(n=6)was less than 5%.Conclusion The method is simple,rapid and accurate,and can be used to detect the 7 essence ingredients in Acanthopanax Cortex.
关键词
气相色谱法/五加皮/掺伪/香精
Key words
gas chromatography/Acanthopanacis Cortex/adulteration/essence
维普
万方数据