Objective To establish a method for the simultaneous determination of syringin,neochlorogenic acid,chlorogenic acid,cryptochlorogenic,costunolide and dehydrocostus lactone in Vladimiriasouliei Formula Granules by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS),and to evaluate their quality comprehensively.Methods An Agilent SB C18 column(100 mm×2.1 mm,1.8 μm)was used to separate the samples with gradient elution of acetonitrile-3 mL·L-1 formic acid as mobile phase,flow rate of 0.3 mL·min-1,column temperature of 35℃and the injection volume of 5 μL.The 6 compounds were quantitatively analyzed with electrospray ionization(ESI)ion source and multiple-reaction monitoring(MRM)scanning in positive and negative ions mode,simultaneously.Results The 6 components of Vladimiriasouliei Formula Granules showed good linear relationship(r≥0.997 0)with their peak areas in their respective concentration ranges,with respective RSDs of precision less than 2.56%,RSDs of repeatability less than 2.15%and the RSDs of stability less than 3.62%.The average recoveries were between 96.23%and 103.41%.Conclusion The method is sensitive,accurate,and reproducible which can be used for the determination of the 6 components in Vladimiriasouliei Formula Granules and the improvement of the quality standard of Vladimiriasouliei.
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