Objective To establish an ultra performance liquid chromatography(UPLC)method for the determination of paracetamol,caffeine and chlorphenamine maleate in Paracetamol,Caffeine and Chlorphenamine Tablets.Methods ACQUITY UPLC® HSS T3 column(100 mm×2.1 mm,1.8 μm)was used,and the mobile phase was methanol and glacial acetic acid containing 2 mL·L-1 triethylamine(glacial acetic acid∶water=65∶1.5)(38∶62).The column temperature was 35 ℃,and the flow rate was 0.2 mL·min-1.The detection wavelength was set at 262 nm.Results The linear ranges of acetaminophen,caffeine and chlorphenamine maleate were 100.37-401.47,6.66-33.28 and 6.55-45.82 μg·mL-1 respectively,and the correlation coefficients(r)were all bigger than 0.999.The RSDs of stability and repeatability tests were all less than 2.0%,and the average recoveries(n=9)of the 3 components were 99.61%,103.95%and 99.06%respectively.Conclusion The UPLC method established in this paper has more advantages in the quality control of Paracetamol,Caffeine and Chlorphenamine Tablet,compared with the current drug implementing standard.
关键词
超高效液相色谱法/氨酚咖那敏片/对乙酰氨基酚/咖啡因/马来酸氯苯那敏
Key words
UPLC/Paracetamol,Caffeine and Chlorphenamine Tablets/acetaminophen/caffeine/chlorphenamine maleate
维普
万方数据