Content determination of the 3 major components in Paracetamol,Caffeine and Chlorphenamine Tablets by UPLC
Objective To establish an ultra performance liquid chromatography(UPLC)method for the determination of paracetamol,caffeine and chlorphenamine maleate in Paracetamol,Caffeine and Chlorphenamine Tablets.Methods ACQUITY UPLC® HSS T3 column(100 mm×2.1 mm,1.8 μm)was used,and the mobile phase was methanol and glacial acetic acid containing 2 mL·L-1 triethylamine(glacial acetic acid∶water=65∶1.5)(38∶62).The column temperature was 35 ℃,and the flow rate was 0.2 mL·min-1.The detection wavelength was set at 262 nm.Results The linear ranges of acetaminophen,caffeine and chlorphenamine maleate were 100.37-401.47,6.66-33.28 and 6.55-45.82 μg·mL-1 respectively,and the correlation coefficients(r)were all bigger than 0.999.The RSDs of stability and repeatability tests were all less than 2.0%,and the average recoveries(n=9)of the 3 components were 99.61%,103.95%and 99.06%respectively.Conclusion The UPLC method established in this paper has more advantages in the quality control of Paracetamol,Caffeine and Chlorphenamine Tablet,compared with the current drug implementing standard.
UPLCParacetamol,Caffeine and Chlorphenamine Tabletsacetaminophencaffeinechlorphenamine maleate
万方数据
维普
