Study on the quality standard of Tibetan medicine Qiwei Xuebing Pills
Objective To establish the quality standard of Tibetan medicine,Qiwei Xuebing Pills.Methods Radix Aucklandiae in the prescription was identified by thin-layer chromatography(TLC).The contents of gallic acid,coumarin,and iso-coumarin in the pills were determined by high performance liquid chromatography(HPLC).The chromatographic column was packed with C18.The mobile phase was acetonitrile 1 mL·L-1 phosphoric acid solution,in gradient elution mode.The flow rate was 1 mL·min-1,The detection wavelength was 225 nm and the injection volume was 5 µL.Results TLC spots were clear and negative samples showed no interference.There were good linear relationship between the mass concentrations of gallic acid,coumarin,and isoquercetin with their peak areas in the ranges of 0.892-4.461,0.975-4.874,and 0.956-4.780 mg·mL-1,respectively.Conclusion The method exhibited satisfactory specificity and sensitivity,also being user-friendly.The method is suitable for both qualitative and quantitative analysis of Tibetan medicine,Qiwei Xuebing Pills.This provides reference data and experimental basis for the research and improvement of the quality standards of this product.
万方数据
维普
