Effect of Yinma Jiedu Granules on regulating lung inflammation in acute lung injury mice
Objective To analyze the effect and safety of Yinma Jiedu Granules on regulating lung inflammation in laboratory-bred strain(BALB/c)acute lung injury mice.Methods 70 C57BL/6J male mice of 6-8 weeks old were selected to construct BALB/c acute lung injury inflammation model by using lipopolysaccharide(LPS),and the other 10 C57BL/6J male mice of 6-8 weeks old were used as blank group A for the control.The 70 model mice were randomly divided into 7 groups(model group B,low-dose prophylactic treatment group C1 of Yinma Jiedu Granules,high-dose prophylactic treatment group C2 of Yinma Jiedu Granules,low-dose treatment group D1 of Yinma Jiedu Granules,high-dose treatment group D2 of Yinma Jiedu Granules,low-dose continuous treatment group E1 of Yinma Jiedu Granules,and high-dose continuous treatment group E2 of Yinma Jiedu Granules),with 10 mice in each group.The drug toxicity,alveolar lavage fluid cell count,inflammatory factors and hematoxylin-eosin(HE)staining of lung tissue in each group were compared.Results During the modeling process,a total of 6 mice died,with a success rate of 92.11%.Compared with the control group,the number of inflammatory cells,inflammatory factors,and HE staining infiltration rate of inflammatory cells in the alveolar lavage fluid of the model group were all increased(P＜0.05).Compared with normal lung tissue,HE staining of lung tissue in acute lung injury model mice showed alveolar septal congestion,edema,and infiltration of a large number of inflammatory cells.The cell count and inflammatory cytokine levels in the alveolar lavage fluid of successfully modeled mice were higher than those in Group A,and the differences were statistically significant(P＜0.05).The state of the model group mice was poor,characterized by towering and clumping hair,significantly reduced food and water intake,reduced body weight,and dry stools.After administration of Yinma Jiedu Granules orally for 1 day to each group of acute lung injury model mice,the C2,D2,and E2 groups of mice were in good condition.After 8 days of modeling,the mortality rate of model group(Group B)mice reached 30%,significantly higher than other groups(P＜0.05);Pathologically,compared with normal lung tissue,the HE staining of lung tissue in acute lung injury model mice showed congestion and edema of alveolar septa,as well as infiltration of a large number of inflammatory cells.After administration of Yinma Jiedu Granules,the congestion,edema,and infiltration of inflammatory cells in the alveolar septa were significantly reduced,and the above effects were proportional to the dosage of Yinma Jiedu Granules.In terms of cell count in alveolar lavage fluid,compared with group A,group B mice showed a significant increase in white blood cell,neutrophil,and lymphocyte counts.Compared with mice in Group E and Group B,the 3 types of cell counts in Group C and Group D of the treatment group were significantly reduced(P＜0.05);And the counts of 3 types of cells in the C2,D2,and E2 groups of mice were lower than those in the C1,D1,and E1 groups,respectively(P＜0.05).In terms of inflammatory factors,compared with Group A,Group B showed the levels of IL-6,TNF-α and IL-1β all significantly increased(P＜0.05).Compared with Group B,the levels of IL-1β in C1,D1,and E1 groups decreased,while the levels of TNF-α and IL-1β in the C2,D2,and E2 groups of mice decreased(P＜0.05);And the IL-6,TNF-α and IL-1β in C2,D2,and E2 groups of mice were lower than those in C1 group,D1 group,and E1 group(P＜0.05).Conclusion Yinma Jiedu Granules have a significant protective effect on BALB/c acute lung injury,and can reduce the activation of inflammatory cells and inflammatory factors in lungs,thereby alleviate the symptoms of acute lung injury.
万方数据
维普
