Effects and mechanisms of the extract of Lysimachia chinensis L.on renal injury caused by chemotherapy in rats with gastric cancer
Objective To explore the effect of the extract of Lysimachia chinensis L.(ELC)on renal injury in rats with gastric cancer after chemotherapy,and to explore the possible mechanism.Methods Fifty SD rats were selected to establish gastric cancer model,and 10 of the 40 successfully modeled rats were randomly selected as gastric cancer group,and the rest were randomly divided into injury group,intervention group and ELC combined with nuclear factor-E2 related factor2(Nrf2)inhibitor(ML385)group,with 10 rats in each.The intervention group was administered with 800 mg·kg-1 of ELC,the ELC combined with ML385 group was administered with 800 mg·kg-1 of ELC and 20 mg·kg-1 of ML385,the gastric cancer group and the injury group were administered with equal volume of normal saline.Once a day for 1 week.The serum urea nitrogen(BUN)and serum creatinine(CysC)levels were detected.The kidney mass index was calculated.The malondialdehyde(MDA),superoxide dismutase(GSH-Px)levels,the protein expression of nuclear factor E2 related factor 2(Nrf2)and heme oxygenase 1(HO-1)in kidney tissue were detected.Results Compared with the injury group,the levels of CysC,BUN,kidney mass index,and MDA in the intervention group were decreased,of which the activities of GSH-Px were increased(P＜0.05).Compared with the intervention group,the levels of CysC,BUN,kidney mass index,and MDA in the ELC combined with ML385 group were increased,of which the activities of GSH-Px were decreased(P＜0.05).Compared with the injury group,the expression of Nrf2 and HO-1 protein in the intervention group were increased(P＜0.05).Compared with the intervention group,the expression of Nrf2 and HO-1 protein in the ELC combined with ML385 group were decreased(P＜0.05).Conclusion The extract of ELC may act through activating the Nrf2/HO-1 signaling pathway to reduce the oxidative stress and kidney damage in the kidney tissue of rats with gastric cancer chemotherapy,and to protect the renal function.
extract of Lysimachia christinae L.gastric cancerchemotherapykidney injuryoxidative stress
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