Effects of volatile oil from Acorus tatarinowii on lung injury in neonatal rats induced by intrauterine infection
Objective To investigate the effect and mechanism of volatile oil of Acorus tatarinowii(VOAT)on lung injury induced by intrauterine infection in neonatal rats.Methods Six 15-days pregnant SD rats were randomly selected as the control group.The test rats were injected with E.coli into cervix to construct the intrauterine infection model.The modeled rats were randomly divided into model group,low,medium and high dose VOAT groups,with 6 rats in each group.The drugs were administered the next day.The oral doses were 5,10 and 20 g·kg-1·d-1 respectively,continuous administration until delivery.The rats in the model group and the control group were only given the same amount of normal saline.The gestational age of newborn rats in each group was recorded.The lung index of newborn rats was measured.The levels of serum interleukin(IL)-21,IL-6 and IL-1β in neonatal rats were detected by enzyme-linked immunosorbent assay(ELISA).The pathological changes of lung tissue were detected by hematoxylin and eosin(HE)staining.The relative expression levels of mRNA of IL-21 and IL-21R in lung tissue were detected by quantitative real-time PCR(RT-qPCR).The relative expression of IL-21,IL-21R,STAT3 and p-STAT3 protein in lung tissue was detected by western blot.Results Compared with the control group,the gestational age of newborn rats was shortened,the lung index was decreased,and the serum IL-21,IL-6 and IL-1β,the relative expression of mRNA of IL-21,IL-21R and the relative expression of IL-21,IL-21R and p-STAT3 protein in lung tissue were increased in the model group and the 3 VOAT administration groups(P＜0.05),and there were pathological changes such as inflammatory cell infiltration,thickening of alveolar wall,enlargement of alveolar cavity and structural disorder.Compared with the model group,the lung index was increased,the serum IL-21,IL-6 and IL-1β,the relative expression of mRNA of IL-21,IL-21R and the relative expression of IL-21,IL-21R and p-STAT3 protein in lung tissue were all decreased in the low,medium and high dose VOAT groups(P＜0.05),and the pathological injury of lung tissue was relieved to varying degrees.The effects of VOAT on neonatal lung injury induced by intrauterine infection were dose-dependent(P＜0.05).Conclusion VOAT could alleviate lung injury in neonatal rats caused by intrauterine infection,and its mechanism may be related to inhibiting the activation of IL-21/STAT3 pathway and reducing the level of inflammation.
volatile oil of Acorus tatarinowiiIL-21/STAT3 pathwayintrauterine infectionlung injurynewborn rat
万方数据
维普
