Effect of psoralen on invasion and metastasis of osteosarcoma cells through miR-101/PI3K/Akt axis
Objective To investigate the inhibitory effect of psoralen on the invasion and metastasis of osteosarcoma cells and to explore its possible mechanism.Methods Psoralen with different concentrations acted on human osteosarcoma cells(MG-63).The cell survival was detected by CCK-8 method to screen the best inhibitory concentration.The relative expression of miR-101 in osteosarcoma and normal tissues was detected by real-time fluorescence quantitative method.The logarithmic growth MG-63 cells were randomly divided into control group,miR-NC group,miR-101 group,psoralen group,and psoralen combined with miR-101 group.The inhibition rate of cell proliferation was detected by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT)assay,the ability of cell invasion and migration was detected by Transwell chamber test,and the mRNA expression of phosphatidylinositol 3-kinase(PI3K)and protein kinase B(Akt)was detected by quantitative real time polymerase chain reaction(RT-PCR).The expressions of phosphorylated myophosphatidylinositol 3-kinase(p-PI3K),phosphorylated protein kinase B(p-Akt),matrix metalloproteinase-2(MMP-2)and matrix metalloproteinase-9(MMP-9)were detected by Western blotting.Results Compared with the control group,the cell proliferation inhibition rate was increased,and the number of cell invasion,cell migration,PI3K and Akt mRNA,p-PI3K,p-Akt,MMP2 and MMP9 protein expression levels were decreased in miR-101 group,psoralen group,and psoralen combined with miR-101 group(P＜0.05).Compared with miR-101 group and psoralen group,the cell proliferation inhibition rate was increased,and the number of cell invasion,cell migration,PI3K and Akt mRNA,p-PI3K,p-Akt,MMP-2 and MMP-9 protein expression levels were decreased in psoralen combined with miR-101 group(P＜0.05).Conclusion Psoralen can reduce the proliferation of osteosarcoma cell line MG-63 and inhibit its invasion and migration.Its mechanism may be related to regulating the level of miR-101 and affecting PI3K/Akt signaling pathway.
psoralenmiR-101human osteosarcoma cellsmyophosphatidylinositol 3-kinaseprotein kinase B
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