Study on the preparation and anti-colon cancer activity of hydroxycamptothecin loaded ferritin inclusion complex
Objective In order to increase the solubility and improve the therapeutic effect of colon cancer,a hydroxycamptothecin(HCPT)loaded ferritin(Fn)inclusion complex(hydroxycamptothecin@ferritin,HCPT@Fn)was prepared.Methods Dialysis method was used to prepare HCPT@Fn.Then,the particle size and Zeta potential of HCPT@Fn were evaluated by laser particle size analyzer.The drug loading of HCPT@Fn was determined by ultra-high performance liquid chromatography(UPLC).The cytotoxicity of HCPT@Fn on human colon cancer cells(HT-29)cells was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay,alive/dead cell staining test and clone formation assay.The uptake of HCPT@Fn in HT-29 cells was detected by fluorescence microscope.The safety of HCPT@Fn was investigated by hemolysis test and HE staining preliminarily.Results The particle size and Zeta potential of HCPT@Fn were 148.4 nm and-34.4 mV,respectively.The drug loading of HCPT in HCPT@Fn was 37.46%.HCPT@Fn can accumulate in large amounts in HT-29 cells,inhibiting the proliferation and clone formation of HT-29 cells significantly.In the set concentration range,HCPT@Fn did not cause significant hemolysis,and did not cause significant damage to normal tissue in mice.Conclusion HCPT@Fn could increase the solubility of HCPT,enhance the anti-colon cancer activity of HCPT and reduce toxic side effects.
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