Effect of Rupi Sanjie Capsules on rat goiter induced by propylthiouracil
Objective To observe the effect of Rupi Sanjie Capsules on propylthiouracil(PTU)induced goiter in rats.Methods Sixty SD rats were randomly divided into control group,model group,levothyroxine sodium positive group(0.02 mg·kg-1),Rupi Sanjie Capsules low,medium and high dose groups(0.5,1,2 g·kg-1),with 10 rats in each group,half male and female.All groups,except for the control group,were administered by gavage with 10 mg·kg-1 PTU to establish the pathological model of goiter,once a day for 2 weeks.Two weeks later,all rats in each group except for the control group were given PTU once every 2 days to maintain the pathological changes.The positive group and Rupi Sanjie group were given different drugs once a day for 4 weeks.The body weight was observed every week.At week 6,the blood was collected from the femoral artery of the rats following their last administration.Thyroid glands were dissected and weighed,and the thyroid coefficient was calculated.The contents of free triiodothyronine(FT3),free tetraiodothyronine(FT4)and thyroid stimulating hormone(TSH)in serum were determined by enzyme linked immunosorbent assay(ELISA).The histopathological changes were observed by HE staining.Results Within 6 weeks,the body weight of rats in the normal control group increased normally;Compared with the control group,the weight gain of the model group was significantly slowed down(P<0.05),while compared with the model group,the body weight growth of the low,medium and high dose Rupi Sanjie groups was improved,especially the body weight of the female rats recovered to the same level as the control group.The thyroid coefficients of rats in the control group were in the normal physiological range,while those in the model group were significantly increased(P<0.01).Compared with the model group,the thyroid index of the drug improvement group was significantly decreased(P<0.05).Compared with the control group,the levels of FT3 and FT4 in the model group were slightly lower,and the level of TSH was slightly higher,which tended to be disordered.Compared with the model group,the levels of FT3,FT4 and TSH in the Rupi Sanjie low,medium and high dose groups tended to normal physiological values after 4 weeks of treatment.HE staining showed that the thyroid structure of the model group was obviously damaged,the epithelial cells were significantly proliferated,some follicles were vacuolated,the papillary body protrude into the bubble cavity,the size of the bubble was uneven,and the colloid content was reduced.Compared with the model group,the thyroid follicular structure was significantly improved,epithelial cell swelling and mastoid hyperplasia were reduced in the test drug groups,and the atrophy of thyroid follicular structure was significantly improved,the follicular cavity was enlarged,and epithelial cell proliferation was improved in the high and medium dose Rupi Sanjie Capsules groups.Conclusion Rupi Sanjie Capsules,administered by gavage at a dosage of 1-2 g·kg-1 for a continuous period of 4 weeks,exerts a therapeutic efficacy against PTU-induced goiter in rats.
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