Extraction and Anti-tumor Activity Analysis of Polysaccharides from Lycium Ruthenicum
To study the optimal extraction process conditions and anti-tumor activity of polysaccharides from lycium ruthenicum,orthogonal experiments were designed by changing factors such as material liquid ratio,ex-traction temperature,and extraction time.The extracted lycium ruthenicum polysaccharide solution was treated with alcohol precipitation,filtration,and drying to obtain crude polysaccharide powder.The powder was decolor-ized by macroporous resin,deproteinized by Sevage method,and refined to obtain lycium ruthenicum polysaccha-ride.The results showed that the optimal extraction process conditions for polysaccharides from lycium rutheni-cum were material liquid ratio 1∶25,extraction temperature 80℃,extraction time 3 hours,and the polysaccha-ride extraction rate was 11.58%;Lycium ruthenicum polysaccharides have an inhibitory effect on the prolifera-tion of human chronic myeloid leukemia cell line K562 in vitro,and are expected to become an ideal anti-tumor adjuvant.
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