Effect and mechanism of long-chain non-coding RNA467 on the proliferation and inva-sion of endometrial carcinoma by regulating SATB1 activity
Objective:To investigate whether long-chain noncoding RNA467 affects the progression of endometrial cancer by regulating SATB1 and activating PI3K/Akt pathway.Methods:Firstly,through the search(http://starbase.sysu.edu.cn)found target mRNA(SATB1)of LINC00467,normal endometrial cells,HEC-1A and Ishikawa cell were cultured.Transfection sequence experiments,RT-qPCR,Western blot and other methods were used to confirm that LINC00467 affected PI3K/Akt through the regulation of SATB1,thus promoting the proliferation,migration and invasion of endometrial cancer cells.Results:After knockdown the expression of LINC00467,the expression of SATB1 decreased,and the difference was statistical-ly significant(P＜0.01).After SATB1 inhibition,the expressions of p-Akt and P-PI3K de-creased,and the differences were statistically significant(P＜0.01).After transfection with pcDNA3.1-SATB1,the expressions of p-Akt and P-PI3K were increased,and the differences were statistically significant(P＜0.01).Compared with the sh-NC group,the expression levels of p-Akt and p-PI3K in sh-LINC00467#1 and sh-LINC00467#1+pcDNA3.1 groups were sig-nificantly decreased,the number of EdU positive cells was significantly decreased,the number of cell clones was significantly decreased,and the expression level of Bcl-2 protein was signifi-cantly decreased,cleaved caspase 3 protein expression was significantly increased,apoptosis rate was significantly increased,cell mobility was significantly decreased,and the number of invasive cells was significantly decreased,with statistical significance(P＜0.01).In the sh-LINC00467#l+pcDNA3.1-SATB1 group,the expression levels of p-Akt and p-PI3K,the number of EdU positive cells,the number of cell clones,the protein expression levels of Bcl-2 and cleaved caspase 3,the apoptosis rate,cell mobility,and the number of invasive cells were not significant-ly decreased.There were no significant differences(P＞0.05).Conclusion:LINC00467 can promote the proliferation,migration and invasion of endometrial cancer cells and inhibit their apoptosis by promoting the expression of SATB1 and then activating PI3K/Akt pathway.
万方数据
维普
