Preparation and antibacterial properties of loaded aggregation-induced emission photosensitizers nanofiber membranes
Photodynamic antibacterial fibers can generate reactive oxygen species(ROS)under light exposure,rapidly reacting with microorganisms,including Gram-positive and Gram-negative bacteria,fungi,viruses,etc.,so as to achieve sterilization effects.In the field of medical protective textiles,such highly efficient,low-toxicity,and low-resistance antimicrobial materials play a crucial role.However,traditional photodynamic antibacterial fibers face challenges such as low ROS production rates and leakage of photosensitizers(PS).In this study,the AIE molecule with photosensitizer properties,(3-cyano-5,5-dimethyl-4-(4-(1,2,2-triphenylvinyl)styryl)furan-2(5H)-ylidene)malononitrile(TPE-TCF),which possesses a donor-acceptor(D-A)structure,was used as the photosensitizer.By blending it with polyacrylonitrile(PAN)and the hydrophilic polymer polyvinylpyrrolidone(PVP),TPE-TCF@PAN/PVP,a nanofiber membrane with excellent hydrophilicity,was prepared by using electrospinning technology.The study explored the effects of TPE-TCF doping level on the wettability,ROS production rate,and antibacterial properties of the nanofiber membrane.Proton nuclear magnetic resonance spectroscopy(1 H-NMR)confirmed the synthesis of TPE-TCF molecules,and UV-Vis spectrophotometry and fluorescence spectroscopy demonstrated that the aggregation-induced emission(AIE)property of TPE-TCF facilitated the generation of total ROS.Fourier transform infrared spectroscopy(FTIR)analysis confirmed the successful loading of TPE-TCF into the nanofiber membrane.Scanning electron microscopy(SEM)and confocal laser scanning microscopy(CLSM)showed that the TPE-TCF@PAN/PVP nanofiber membrane exhibited a regular morphology,and TPE-TCF molecules were aggregated and distributed in the nanofiber membrane.The loading stability of TPE-TCF was also evaluated by measuring the UV absorption of the leach solution from the nanofiber membrane.The results indicated excellent loading stability of TPE-TCF in the nanofiber membrane.Water contact angle(WCA)experiments demonstrated that the hydrophobicity of the nanofiber membrane increased with the increase of TPE-TCF doping levels.ROS production rates of nanofiber membrane with different TPE-TCF doping levels were evaluated by using indicators such as ABDA and DCFH,revealing that the TPE-TCF@PAN/PVP nanofiber membrane with a TPE-TCF mass fraction of 0.4%exhibited the optimal ROS production efficiency.Finally,the antimicrobial performance of the nanofiber membrane was assessed.The results showed that the TPE-TCF@PAN/PVP nanofiber membrane with a TPE-TCF mass fraction of 0.4%demonstrated the best bacteriostatic effect,achieving inhibition efficiencies of 96.2%for S.aureus and 100%for E.coli under 20 minutes of white light irradiation at ultra-low power.In summary,this study successfully prepared a TPE-TCF@PAN/PVP nanofiber membrane with highly efficient broad-spectrum antibacterial properties using electrospinning technology.The research offers a new approach to the development of medical protective materials and has the potential to address issues related to cross-infection.
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