Objective To investigate the expression profile of circRNA in nuclear receptor NURR1 overexpressed prostate cancer(PCa)cells,so as to provide reference for revealing the mechanism of PCa progression.Methods The expression of NURR1 in PCa was analyzed with UALCAN and TNMplot.The distinct circRNAs in NURR1 overexpressed PCa cells were screened with RNA-sequencing.The functions and signal pathways of differentially expressed circRNA molecules were analyzed with GO and KEGG.Results The circ_0000915 was significantly downregulated in DU145,LNCaP and PC3 cells.In NURR1 overexpressed DU145 cells,circ_0005991 was up-regulated,while circ_0001460 and circ_0001315 were down-regulated.In NURR1 overexpressed LNCaP cells,circ_0040729 and circ_0000722 were significantly up-regulated.In NURR1 overexpressed PC3 cells,circ_0001577,circ_0000854 and circ_0018168 were up-regulated,while circ_013035,circ_0003028,circ_0082096 and circ_0005320 were down-regulated.KEGG analysis revealed that the differentially expressed circRNAs were significantly associated with dorsal/ventral neural tube patterns,protein folding chaperones,disordered domain specific binding,positive regulation of BMP signaling pathways,and neural tube patterning functions.Conclusion CircRNAs play an important role in NURR1 mediated PCa progression,but there are certain differences among different prostate cancer cell types.The regulatory mechanism between NURR1 and circ_0000915 in the progression of PCa needs further investigation.
关键词
核受体/NURR1/前列腺癌/环状RNA/侵袭转移/DU145/LNCaP/PC3
Key words
nuclear receptor/NURR1/prostate cancer/circRNA/invasion and metastasis/DU145/LNCaP/PC3
维普
万方数据