The effect and mechanism of SERPINA5 on the migration,invasion ability and chemotherapy sensitivity of esophageal squamous cell carcinoma cells
Objective To explore the effects and mechanisms of SERPINA5 on the migration,invasion ability and chemotherapy sensitivity of esophageal squamous cell carcinoma cells.Methods The expression levels of SERPINA5 in esophageal cancer and adjacent normal tissues were analyzed using the TCGA database.Western blot was used to detect the expression of SERPINA5 in human esophageal mucosal epithelial cell line SHEE and esophageal squamous cell carcinoma cell lines TE-1,ECA109 and KYSE150.KYSE150/TE-1 cells were transfected with overexpression control(oe NC)and overexpression SERPINA5(oe SERPINA5)lentivirus.The half-maximal inhibitory concentration(IC50)of cisplatin(CDDP)at different concentrations(0,20,40,60,80,100μmol/L)for 24 hours was detected by CCK-8 assay.Cell scratch and Transwell experiments were used to detect the migration and invasion ability of cells.Western blot was used to detect the expression levels of Vimentin,E-cadherin and Fn/Integrin β1 signaling pathway related proteins.Results SERPINA5 showed low expression in esophageal cancer tissues and esophageal squamous cell carcinoma cells.Overexpression of SERPINA5 could reduce the migration and invasion ability of esophageal squamous cell carcinoma cells,and increase their sensitivity to cisplatin.SERPINA5 could inhibit the expression of Vimentin,Fn,Integrin β1 and p-FAK,and promote the expression of E-cadherin.Conclusion SERPINA5 may inhibit epithelial mesenchymal transition of esophageal squamous cell carcinoma cells by regulating the Fn/Integrin β1 signaling pathway,thereby affecting their migration,invasion ability and chemotherapy sensitivity.
万方数据
维普
