Effect of US-FNAC combined with BRAFV600E gene mutation detection on diagnostic efficiency of benign and malignant thyroid nodules
Objective To investigate the effect of ultrasound-guided fine needle aspiration cytology(US-FNAC)combined with BRAFV600E genetic testing on the diagnostic efficiency of benign and malignant thyroid nodules.Methods A total of 160 cases of TI-RADS 4 thyroid nodules in the thyroid image reporting data system were retrospectively analyzed.All cases underwent thyroid US-FNAC examination and BRAFV600E gene test,and the postoperative pathological results served as the contrast.The thyroid nodule was classified according to the Bethesda reporting system.The quantitative fluorescence polymerase chain re-action(qRT-PCR)test was used to detect the BRAFV600E gene mutation.The sensitivity,specificity,accu-racy,positive predictive value and negative predictive value of the single detection and combined detection of the above two methods in the diagnosis of papillary thyroid carcinoma(PTC)were analyzed.Results Among 160 patients with TI-RADS 4 type thyroid nodules,the US-FNAC results showed 22 cases of benign,20 cases of cellular atypical lesions of unclear significance and 118 cases of suspicious papillary carcinoma cells.The sensitivity,specificity and accuracy of US-FNAC diagnosis were 99.11％,75.00％and 92.49％respectively,the positive predictive value was 94.07％and negative predictive value was 95.45％;the BRAVFV600E ge-netic mutation occurred in 112 cases.The sensitivity,specificity and accuracy of BRAFV600E gene mutation detection were 89.29％,92.86％and 90.00％respectively,the positive predictive value was 98.04％and the negative predictive value was 68.42％.The sensitivity,specificity and accuracy of US-FNAC combined with BRAFV600E genetic mutation detection were 100.00％,67.86％and 93.57％,the positive predictive value was 92.56％and the negative predictive value was 100.00％.In the comparison of postoperative histopatho-logical results,the BRAFV600E gene mutation detection could differentially diagnose 20 cases of unclear sig-nificance cell atypical lesions in the US-FNAC results,and the difference was statistically significant(P＜0.05).Conclusion US-FNAC combined with BRAFV600E gene mutation detection could improve the diag-nostic sensitivity of benign and malignant thyroid nodules before surgery,especially it has the high clinical val-ue for atypical cells of unknown significance diagnosed by US-FNAC.
Ultrasound-guided fine needle aspiration cytologyPapillary thyroid carcinomaBRAFV600E gene mutationDiagnostic efficiency
国家科技期刊平台
NSTL
万方数据
