Effect of tumor markers and EMT pathway expression level on prognosis of melanoma
Objective To explore the effect of EMT pathway on the prognosis of the patients with mel-anoma and the correlation between common tumor markers and EMT pathway to provide the new ideas for clinical identification and follow-up of melanoma patients with high risk of metastasis.Methods The tran-scriptional and clinical data of 461 melanoma patients were obtained from the TCGA database.The expression of each sample was analyzed by GSVA package with non-parametric unsupervised analysis,and the expression score of EMT pathway was obtained and divided into the low and high groups.The survival package was used for conducting the survival analysis.Then the mRNA and EMT pathway expression levels of tumor markers conducted the Spearman correlation analysis,moreover the subgroup analysis was performed according to age,sex and tumor stage.The protein expression levels of tumor markers in melanoma patients were further down-loaded from the TCGA database,and the correlation analysis was also conducted to explore the correlation be-tween the common tumor markers and EMT pathways from two perspectives of mRNA and protein expres-sion levels.Finally,the above tumor markers,EMT pathway expression levels and clinical features were in-cluded in a multi-factor analysis to determine their impact on melanoma prognosis.Results The high expres-sion of EMT pathway predicted the poor prognosis of melanoma patients.The mRNA expression levels of melanoma tumor markers PMEL,MLANA,TYR,and MITF were negatively correlated with the EMT path-way(P＜0.05),while the mRNA expression level of MKI67 was positively correlated with the EMT pathway(P＜0.05).The results of subgroup analysis of the correlation between the tumor markers and EMT pathway were basically consistent with those obtained in the total sample.High expression of tumor marker PMEL and low expression of EMT-related pathway predicted a good prognosis,while low expression of MLANA and MKI67 predicted a good prognosis when EMT-related pathway was low expression(P＜0.05).The protein expression levels of PMEL,MLANA and MITF were negatively correlated with the expression of EMT path-way(P＜0.05).PMEL,age and tumor stage might be the independent risk factors for melanoma prognosis.Conclusion The lower expressions of PMEL,MLANA and MITF common markers of melanoma may be re-lated to the high expression of EMT pathway.When the EMT-related pathways are low,high expression of PMEL and MLANA and low expression of MKI67 predict a good prognosis,which should be paid a certain de-gree of attention to during follow-up.
国家科技期刊平台
NSTL
万方数据
