Effect of Ophiopogon japonicus polysaccharides on osteoclast differentiation
Objective To explore the effect of Ophiopogon japonicus polysaccharides(OPS)on osteo-clast differentiation.Methods Bone marrow mononuclear macrophages(BMMs)were extracted from the femur of C57BL/6j mice,and 2.5,10.0 and 40.0 μg/mL OPS were added to the cell culture medium,respectively(groups A,B and C),while no OPS was added to the control group.The effects of OPS on BMMs cell activity,osteoclast bone re-sorption capacity and tartrate-resistant acid phosphatase(TRAP)activity were detected.Meanwhile,the effects of OPS on the mRNA expression of carbonic anhydrase 2(Car2),cathepsin K(CTSK),matrix metalloproteinase 9(MMP-9),TRAP and vacuolar H+-ATPase(V-ATPase)were analyzed.Results There was no significant difference in ab-sorbance of group A,B and C at 48 and 96 h(P＞0.05).Compared with the control group(1.00±0.15),the number of mature osteoclasts in group B was reduced to(0.61±0.06)(P＜0.05),and that in group C was(0.31±0.03)(P＜0.05).Compared with the control group,TRAP activity of osteoclasts in group B and C at 5th day was significantly decreased(P＜0.05).The TRAP activity of osteoclasts in group A,B and C at 7th day was significantly lower than that in control group(P＜0.05).The bone resorption area of group A,B and C was only 71％,40％and 6％of that of the control group(P＜0.05).Compared with the control group,the mRNA expression levels of Car2,CTSK,MMP-9,TRAP,and V-ATPase in group B and C were significantly decreased(P＜0.01).Conclusion OPS can significantly inhibit osteoclast differentiation and bone resorption,which is a potential anti-resorption drug for osteoporosis treatment.
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维普
