Study on whitening and repairing efficacy of nonapeptide-1/ectoin nanoliposomes
Objective To explore the whitening and repairing effects of nonapeptide-1/ectoin nanoli-posomes(Non/Ect-NLPs).Methods The safety of Non/Ect-NLPs was studied by cell viability test,and the uptake of nanoliposomes by melanocytes was observed.Tyrosinase activity,melanin levels,survival rate of ox-idative damage cells,and the levels of human aquaporin-3(AQP3),filaggrin(FLG),and tight junction protein-1(Claudin-1)levels were compared between groups with different levels of Non/Ect-NLPs and free component solution groups.Results The viability of HaCaT cells in both the free component solution group and the groups with different levels of Non/Ect-NLPs were all above 80％.Compared with the free component solu-tion group,when the levels of Non/Ect-NLPs were 250.0 and 500.0 μg/mL,the tyrosinase activity of B16F10 cells was significantly decreased(P＜0.05).Compared with the free component solution group,when the lev-els of Non/Ect-NLPs were 125.0 and 500.0 μg/mL,it could significantly inhibit the secretion of melanin by B1 6F10 cells(P＜0.05).Compared with the model group,Non/Ect-NLPs at 250.0 and 500.0 μg/mL signifi-cantly increased the survival rate of oxidative damaged cells(P＜0.05).Compared with the control group,the free component solution group and the Non/Ect-NLPs group significantly promoted the migration of HaCaT cells(P＜0.01).Compared with the control group,the free component solution group and the Non/Ect-NLPs group had significant increases in AQP3,FLG,and Claudin-1 levels(P＜0.05).Conclusion Non/Ect-NLPs have a good application prospect as a new type of skin whitening and repair efficacy raw material.
Nonapeptide-1EctoinCo-delivery nanocarriersEfficacy of whitening
万方数据
维普
