Study on molecular biological mechanism of Astragalus membranaceus-Codonopsis pilosula in the treatment of gastric cancer based on network pharmacology and molecular docking
Objective To study the molecular biology mechanism of the effective ingredients of Astrag-alus membranaceus-Codonopsis pilosula in the treatment of gastric cancer by network pharmacology and mo-lecular docking.Methods The active ingredients and corresponding targets of Astragalus membranaceus-Codonopsis pilosula were collected from the database of Traditional Chinese Medicine Systems Pharmacology.The targets related to gastric cancer were collected from the Online Mendelian Inheritance in Man and Gene-Cards databases,and the same parts were screened out by comparing with the corresponding targets of drug components,that is,potential targets for overlapping Astragalus membranaceus-Codonopsis pilosula and gas-tric cancer were obtained.The visual Cytoscape3.9.0 software was used to establish the mapping between drugs and disease targets to obtain the action targets of Astragalus membranaceus-Codonopsis pilosula in the treatment of gastric cancer.The STRING software was used to construct the"compound target"action net-work and protein interaction network to screen the key components and key targets that played a role in the treatment of gastric cancer.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analy-sis were used to analyze the potential mechanisms of key targets.Results A total of 63 candidate active ingre-dients of Astragalus membranaceus-Codonopsis pilosula were screened out,and six active ingredients were mainly luteolin,quercetin,kaempferol and isorhamnetin,and 13 main targets were CCND1,MYC,TP53,FOS,MCL1,CDK4 and RUNX2.It mainly focused on biological processes such as the regulation of the mitotic cell cycle,the activation of DNA transcription,and multiple peptidase activities,and mainly focuses on the biologi-cal pathways such as phosphatidylinositol 3 kinase/protein kinase B signaling pathway and P53 signaling path-way.Molecular docking results showed that the active compounds of the drugs had strong binding activity with their corresponding targets.Conclusion The active components of Astragalus membranaceus-Codonop-sis pilosula inhibit the proliferation of gastric cancer cells and promote the apoptosis of cancer cells by regula-ting cell apoptosis and mediating phosphatidylinositol 3 kinase/protein kinase B signaling pathway,which has a multi-component,multi-target and multi-pathway mechanism.
万方数据
维普
