Protective effect of total flavonoids of Sarsaparilla on chronic pelvic inflammatory disease in rats
Objective To investigate the therapeutic effect of total flavonoids of Sarsaparilla on chronic pelvic inflammatory disease(CPID)model rats.Methods CPID rat model were performed by injecting phenol mucilage into the right uterus.The SD rats were divided into the model control group,the high dose group[32.4 g/(kg·d)],the medium dose group[16.2g/(kg·d)],the low dose group[8.1 g/(kg·d)],the Jin-gangteng capsule group,the dexamethasone group according to the random number table method,with ten an-imals each.Rats treated with sham operation were divided into the sham operation group and the background control group according to the random number table method,with ten animals each.In addition,the blank con-trol group(ten animals)was estabished without any treatment.The hemorheology and routine blood indexes of each group were detected 14 days after administration.The morphological changes of the left and right sides of the uterus were observed,the swelling rate and swelling inhibition rate of the uterus were calculated,and the pathological changes of the uterus were observed by hematoxylin-eosin(HE)and Masson staining.The expression of interleukin-1β(IL-1β),IL-4,IL-6 and IL-10 in uterine tissue of rats was detected by enzyme-linked immunosorbent assay(ELISA).Results Compared with the model control group and the Jingangteng capsule group,all dose groups of total flavonoids from Sarsaparilla can significantly reduce the number of white blood cells,lymphocytes,neutrophils,and various hemorheological indicators in the blood of CPID mod-el rats,reduce the rate of uterine swelling in rats,and inhibit the expression of L-1β and IL-6 in rat uterine tis-sue,and promote the expression of IL-4 and IL-10 and can significantly reduce uterine congestion and edema,reduce inflammatory cell infiltration,alleviate fibrous connective tissue proliferation around glands,and im-prove endometrial hyperplasia and fibrosis,with statistical significance(P＜0.01).Conclusion Total fla-vonoids of Sarsaparilla can improve the symptoms of CPID model rats,enhance blood flow,improve local blood circulation,and inhibit uterine inflammation and fibrotic hyperplasia in CPID model rats.
万方数据
维普
