Effect and mechanism of lncRNA Gm44981 on cardiac aging in mice
Objective To analyze the expression level of long non-coding(lnc)RNA Gm44981 during cardiac aging in mice and explore the mechanism of inhibiting cardiac aging.Methods Five 3-month-old young mice and five 24-month-old old mice were selected to extract myocardial tissue RNA,and the expression level of Gm44981 was detected by real-time quantitative polymerase chain reaction(qRT-PCR).In addition,10 rap-idly aging mice aged four months were selected and divided into the control group(AAV-nc)and the Gm44981 overexpression group(AAV-Gm44981 to construct Gm44981 overexpression rapidly aging mouse model),with five mice in each group.The expression levels of Gm44981 and aging-related secretory phenotype(SASP)in the control group and the Gm44981 overexpression group were detected by qRT-PCR,the activity of SA-β-gal was detected by β-galactosidase(SA-β-gal)staining,and the expression of p53 and Sirt1 senes-cence related proteins were detected by western blotting.The expression level of miRNA-34a was detected by qRT-PCR.Results The expression level of Gm44981 in myocardial tissue of old mice was lower than that of young mice,the difference was statistically significant(P＜0.05).Compared with the the control group,the Gm44981 overexpression group showed up-regulated Gm44981 expression,SASP such as interleukin(IL)-1α,IL-6 and SA-β-gal expression were down-regulated,p53 expression was down-regulated,Sirt1 expression was up-regulated,and miRNA-34a expression was down-regulated,with statistically significant differences(P＜0.05).Conclusion The expression of lncRNA Gm44981 is down-regulated during cardiac aging,which may inhibit cardiac aging by acting on miRNA-34a/Sirt1.
HeartAgingLong non-coding RNASenescence related proteinsReal-time quantitative polymerase chain reactionMouse
万方数据
维普
