Macrogenomic analysis of intestinal flora in rats with hepatic encephalopathy after fecal microbiota transplantation
Objective To establish a rat model of hepatic encephalopathy and fecal microbiota trans-plantation(FMT)model,and explore the effects of bacterial transplantation on intestinal flora in rats with he-patic cirrhosis.Methods A total of 30 SD rats were selected and rat liver models were established using car-bon tetrachloride+ethyl alcohol method.The rats were randomly divided into three groups:group A as the normal control group,group B as the hepatic encephalopathy model group,and group C as the FMT group,with 10 rats in each group.Group B was given the same dose of normal saline,and group C was given FMT transplantation(200 μL/d.bacteria 1.5×1011 per mL of bacterial solution).The survival of the rats was observed daily.The intestinal feces of rats were collected for metagenomic sequencing.Results A total of nine rats died during the experiment,while group A did not die.Before and after FMT,the body weight of rats in group C increased significantly after transplanta-tion.Through metagenomic sequencing,it was found that group C could effectively improve the diversity and abun-dance of bacteria compared with group B.Compared with group A,the content of Proteobacteria in group B and C was significantly increased,while the abundance of Bacteroides was significantly decreased.Conclusion FMT can alter the structure of intestinal flora in rats with hepatic encephalopathy.
万方数据
维普
