Research on the medicinal components and potential anticancer mechanism of Herba lsodi Lophanthoidis based on data mining
Objective To explore the medicinal components of Herba lsodi Lophanthoidis and its potential anti-cancer mechanism based on network pharmacology research methods.Methods The chemical components of Herba lsodi Lophanthoidis were collected by literature search,BATMAN-TCM and CancerHSP database,the CAS number was checked by using Chemical Source website,and the active components were screened by TCMSP database and SwissADME online platform.The targets of each active component were predicted by using SwissTargetPrediction on-line platform.Metascape database was used for GO and KEGG analysis and the online mapping platform Bioinformat-ics visualized the results.The STRING platform was used to obtain protein-protein interaction networks and screen key targets,the NIMNT database was used to predict the indications of the key targets.The Cytocape3.9.1 was used to construct"drug-core target-disease"interaction networks.Results A total of 10 active components,including 5,6,3',4-tetrahydroxy-7-methoxyflavone,ferruginol,kaempferol and azaleatin,were screened and they acted on 212 tar-gets.Among them,SRC,PIK3R1,PIK3CA,AKT1,ESR1,CDK1 and EGFR were the core targets.GO functional en-richment analysis obtained 5 310 entries for biological processes,524 entries for cell composition and 981 entries for molecular functions.A total of 260 signaling pathways were captured from KEGG pathway enrichment screening,which were related to cancer,phosphatase D and cGMP-PKGX signaling pathways,etc.Conclusion The anticancer ac-tivity of Herba lsodi Lophanthoidis is the result of the combined action of multi-component,multi-target and multi-pathway,which provides a scientific theoretical basis for the rational clinical use of Chinese herbal medicine in adjuvant treatment of cancer.
万方数据
维普
