Objective To study the effect of Kangfuxin on the proliferation of periodontal tissue-related cells and the anti-inflammatory effect of Kangfuxin in the cell inflammatory response model,and to explore the related mechanism of Kangfuxin in treating periodontitis from the aspects of promoting periodontal tissue regeneration and anti-inflammatory.Methods(1)MTS method was used to detect the effects of different con-centrations of Kangfuxin on the activities of Human Periodontal Ligament Fibroblasts(hPDLFs),human Gin-gival Epithelial Cells(hGECs),THP-1 and MC3T3-E1 proliferation.(2)The cell inflammation model was es-tablished by stimulating mouse macrophage RAW264.7 with bacterial lipopolysaccharide(LPS).The quantita-tive Real-time polymerase chain reaction(qRT-PCR)was used to detect the effects of Kangfuxin on the mR-NA expression of interleukin-1β(IL-1β),IL-10,nitric oxide synthase(NOS)and matrix metalloproteinase-13(MMP-13).Results(1)Compared with the control group:the 0.100 0,0.050 0,0.025 0,0.012 5 mg/mL re-habilitation groups could stimulate the proliferation of hPDLFs,THP-1 and MC3T3-E1 at 24 h and 48 h time points(P＜0.01),and the proliferation promotion effect was concentration-dependent.(2)In the inflammatory cell model,compared with the control group,there was no significant differences in IL-1β and IL-10 mRNA ex-pression levels in the 0.05 mg/mL group and the 0.012 5 mg/mL group(P＞0.05).The mRNA levels of NOS and MMP-13 were decreased,and the difference was statistically significant(P＜0.05).Conclusion Kangfuxin promote the proliferation of hPDLFs,hGECs,THP-1 and MC3T3-E1 in a certain concentration range.Kang-fuxin inhibit LPS-induced inflammatory response of RAW264.7,and its anti-inflammatory mechanism may be the decrease of mRNA expression of NOS and MMP-13.Kangfuxin may treat periodontitis by promoting peri-odontal tissue regeneration and anti-inflammation.
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