Study on the effect of zebrafish on embryo,liver and kidney development of stomatitis mixture based on model organism
Objective To preliminarily analyze the effect of stomatitis mixture on embryo,liver and kid-ney development by using zebrafish as an animal model.Methods When zebrafish developed to 10 h after fer-tilization(10 hpf),they were grouped by simple random method and added with gradient concentration of sto-matitis mixture.After 72 h of drug action,the toxicity of stomatitis mixture on embryonic development and hepat orenal toxicity were observed under microscope and the image data were recorded.Results When the dilution concentration of stomatitis mixture was higher than 1.625 × 10-5g/mL,embryo developmental toxici-ty began to appear in 10hpf zebrafish,showing developmental delay and cardiac cavity stenosis.When the dilu-tion concentration of stomatitis mixture was higher than 6.500 × 10-5 g/mL,it had hepatorenal toxicity to 36 hpf zebrafish.The main manifestations were that the boundary between liver and surrounding tissues was not obvious,and the kidney and swim bladder area increased significantly,accompanied by edema and stasis.Con-clusion The stomatitis mixture has certain embryo and liver and kidney toxicity.Pregnant women,lactating women,and patients with liver and kidney dysfunction,are not allowed to use this product in principle.It must be used with strict limits and careful use under the guidance of doctors.
万方数据
维普
