Exploring the involvement of let-7c-5p in the progression of bronchial asthma by targeting OLFM based on bioinformatics
Objective To explore the involvement of let-7c-5p in the progression of bronchial asthma by targeting OLFM based on bioinformatics.Methods The microarray data sets GSE207751 and GEO222894 in the Gene Expression Omnibus(GEO)database were selected.The"imma"and"DESeq2"software packages of R software were used to analyze the differentially expressed genes(DEGs)and differentially expressed miRNAs in the two data sets.WCGNA analysis was performed using the hclust function.The"ClusterProfil-er"software package was used for gene ontology(GO)functional annotation enrichment analysis and Kyoto Encyclopedia of Genes and Genomes signal pathway enrichment analysis.The STRING database was used to establish a protein interaction network(PPI network),and Cytoscape was used to screen the core genes of pa-tients with severe asthma.The miRWalk,miRBase and miRTarBase databases were used for miRNA predic-tion of target genes.Results A total of 80 DEGS were obtained,including up-regulated OLFM4.After WCG-NA analysis,24 Darked module genes related to the severity of asthma were found.Four core up-regulated genes(including OLFM4)and six miRNAs(including hsa-let-7c-5p)were screened out by PPI grid construc-tion and mRNA-miRNA prediction.A total of 98 differentially expressed miRNAs were obtained,of which five were up-regulated and 93 were down-regulated.Cross-analysis of differential miRNAs between mild asth-ma patients and severe asthma patients showed that let-7c-5p was down-regulated in mild asthma patients and severe asthma patients.Conclusion This study has repeatedly verified that let-7c-5p affects airway inflamma-tion and airway remodeling of asthma by regulating OLFM4 in asthma patients,and participates in the pro-gression of asthma disease,which is conducive to understanding the potential molecular mechanism of the pro-gression of asthma disease.At the same time,it has verified the potential of let-7c-5p as a potential biomarker for asthma,which provides a theoretical basis for further study of the progression of asthma disease.
万方数据
维普
