Screening study of monoclonal antibodies with high affinity to TPBG based on high-throughput surface plasmon resonance technology
Objective To investigate a method of rapidly screen monoclonal antibodies with high affini-ty to trophoblastic glycoprotein(TPBG)from the prepared monoclonal antibodies.Methods High-through-put surface plasmon resonance(SPR)technology was used to immobilize TPBG of different species on the GLC sensor chip by amino coupling.Gradient dilution of the antibody was used as the flow phase to screen monoclonal antibodies that can highly affinity TPBG,and the kinetic constants of the interaction were deter-mined.Antibody 1 binds to human and cynomolgus monkey TPBG with affinity of 65.0 and 31.6 nmol/L,re-spectively.Antibody 6 binds to human,mouse and cynomolgus monkey TPBG with affinity of 77.6,92.1 and 87.7 nmol/L,respectively.Results This study successfully screened two monoclonal antibodies that can spe-cifically bind to TPBG.Meanwhile,the specific binding of these two antibodies to TPBG protein was stable by binding kinetic.Conclusion The monoclonal antibodies with high affinity to TPBG protein are screened out by SPR,which provides a method for the development of some new anti-tumor drugs.
TPBG proteinHigh-throughputSurface plasmon resonanceAffinityMono-clonal antibodiesAnti-tumor drug
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