Effect of TACI fusion protein on renal damage in rats with immunoglobulin A nephropathy
Objective To investigate the effect of transmembrane activator and calcium modulator cy-clophilin ligand interactor-immunoglobulin(TACI-Ig)on renal damage in rats with IgA nephropathy(IgAN).Methods A total of 24 SD rats were randomly divided into the normal control,the model,the TACI-Ig,and the drug control groups with six rats per group.The normal control and model groups received saline,the TACI-Ig group received TACI-Ig(14.36 mg/kg),and the drug control group received prednisolone acetate(5 mg/kg).Twenty-four-hour urinary protein(24h-UP),serum creatinine(Scr),blood urea nitrogen(BUN),ga-lactose-deficient IgA1(Gd-IgA1),Toll-like receptor 4(TLR4),Myeloid differentiation factor 88(MyD88),Nu-clear factor kappa B(NF-κB)protein and mRNA levels were compared among groups to analyze the influence of TACI-Ig on renal damage.Results The 24h-UP levels in the model group were significantly higher than those in the normal control group,while levels in the TACI-Ig and drug control groups were significantly lower than those in the model group(P＜0.05).Serum CRE levels in the TACI-Ig group were lower than those in the model group(P＜0.05).Deposition of immune complexes in the mesangial area of renal tissues was significantly inhibited in the TACI-Ig and drug control groups.Serum Gd-IgA1 levels in the model group were higher than those in the normal control group but lower than those in the TACI-Ig group(P＜0.01).Se-rum Gd-IgA1 levels in the drug control group were lower than those in the model group but higher than those in the TACI-Ig group(P＜0.01).TLR4,MyD88,and NF-κB protein levels in the TACI-Ig group were lower than those in the model group(P＜0.05).TLR4,MyD88 protein levels in the drug control group were higher than those in the TACI-Ig group(P＜0.05).TLR4,MyD88,and NF-κB mRNA levels in the TACI-Ig group were lower than those in the model group(P＜0.05).Conclusion TACI-Ig exhibits therapeutic efficacy in IgAN,improving renal function in rats and inhibiting mesangial matrix expansion and mesangial cell proliferation.
Transmembrane activator and calcium modulator cyclophilin ligand interactorImmuno-globulin A nephropathyToll-like receptorRenal function
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