Expression and regulation of miR-451 and target genes in non-small cell lung cancer
Objective To explore the expression of miR-451 and target gene PSMB8 in non-small cell lung cancer and their correlation with related cell cycle proteins,so as to provide new targets for the treatment of non-small cell lung cancer.Methods The tissue specimens from six lung cancer patients who underwent radical lung cancer surgery in the thoracic surgery department of the hospital from January 2020 to December 2022 were selected,and divided into two groups based on cancer tissue and adjacent tissue,with six samples in each group.The relative expression levels of miR-451,proteasome subunit beta 8(PSMB8),Cyclin D1,Cyclin E1,cyclin dependent kinase 4(CDK4),and CDK2 proteins between the two groups were compared,and the correlation between miR-451 and related cyclin proteins was analyzed.Results The CT value of miR-451 in the lung cancer tissue group was higher than that in the adjacent tissue group,and the difference was statisti-cally significant(P＜0.05).The 2-△△CT value of miR-451 in the lung cancer tissue group was 0.53,and the rel-ative expression level of miR-451 in the adjacent tissue group was 1.87 times higher than that in the lung cancer tissue group.The relative expression level of PSMB8 in the lung cancer tissue group was(1.55±0.23),significantly higher than that in the adjacent tissue group(0.98±0.15),and the difference was statistically significant(P＜0.05).The relative expression levels of Cyclin D1,Cyclin E1,CDK4,and CDK2 proteins in the lung cancer tissue group were significantly higher than those in the adjacent tissue group,with statistical sig-nificance(P＜0.05).The relative expression level of miR-451 was negatively correlated with the relative ex-pression levels of Cyclin D1,Cyclin E1,CDK4,and CDK2 proteins(r=-0.93,-0.89,-0.86,-0.84,P＜0.05).Conclusion Low expression of miR-451 in lung cancer tissue can reduce the inhibitory effect on the ex-pression of target gene PSMB8,thereby interfering with the expression of cell cycle related proteins,affecting cell proliferation and apoptosis,and indirectly regulating the occurrence and development of non-small cell lung cancer.
Cell cycle proteinTarget genesNon-small cell lung cancer
万方数据
维普
