Analysis of multiple pathogens monitoring results of patients with fever and respiratory tract infection in Hechuan district,Chongqing
Objective To analyze the epidemiological characteristics of pathogens in patients with fever and respiratory tract infection in Hechuan district,Chongqing.Methods From 2022 to 2023,a total of 246 pa-tients with fever and acute respiratory tract infection in a hospital in Hechuan District were selected to detect 18 pathogens such as influenza A/B virus,novel coronavirus(SARS-CoV-2)and respiratory syncytial virus(RSV)by multiple polymerase chain reaction(PCR).At the same time,real-time fluorescent quantitative PCR was used to detect SARS-CoV-2,influenza virus and their subtypes.The situation of respiratory pathogen in-fection was analyzed,and the consistency of the two detection methods was compared.Results Among the 246 patients,159 patients were positive for respiratory pathogens,of which 144(58.54％)were infected with one or more pathogens.There was no statistically significant difference in the total detection rate of respirato-ry pathogens among the patients of different genders(P＞0.05).There was no statistically significant differ-ence in the detection rates of various respiratory pathogens among the patients of different genders(P＞0.05).The total detection rate of respiratory pathogens in the patients of different age groups was compared,and the difference was statistically significant(P＜0.05).The detection rates of influenza virus and RSV in the patients of different age groups were statistically significant(P＜0.05),while the detection rates of other re-spiratory pathogens were not statistically significant(P＞0.05).The total detection rate of respiratory patho-gens in the patients visiting different seasons was compared,and the difference was statistically significant(P＜0.05).The detection rates of H3N2+SARS-CoV-2,H1N1,SARS-CoV-2 in the patients visiting different seasons were statistically significant(P＜0.05),while the detection rates of other respiratory pathogens were not statistically significant(P＞0.05).For SARS-CoV-2 and influenza viruses,the sensitivity of multiplex PCR technology and real-time fluorescence quantitative PCR method was basically the same,and the two had good consistency.Conclusion Influenza virus is the main pathogen causing fever with respiratory infections in Hechuan district from 2022 to 2023,and mixed infection with other pathogens is not uncommon.For SARS-CoV-2 and influenza viruses,the sensitivity of multiplex PCR and real-time fluorescence quantitative PCR methods is basically the same.
万方数据
维普
