Antibacterial effect of Coptis chinensis extract on multidrug-resistant Acinetobacter baumannii and its effect on biofilm
Objective To investigate the antibacterial effect of Coptis chinensis extract on multidrug-re-sistant Acinetobacter baumannii(MDR-Ab)and its effect on cell membrane,and to provide theoretical basis for the treatment of MDR-Ab by Coptis chinensis extract.Methods In May,2023,the minimum inhibitory concentration(MIC)of Coptis chinensis extract on MDR-Ab was determined by Oxford cup method.The structural damage of MDR-Ab was observed by scanning electron microscope.The effect of Coptis chinensis extract on MDR-Ab biofilm was observed by propidium iodide staining.The extracellular and extracellular a-denosine triphosphate(ATP)contents of Coptis chinensis extracts were detected after MDR-Ab treatment.Results The MIC of Coptis chinensis extract on MDR-Ab was 64 mg/mL.The morphology of MDR-Ab trea-ted with Coptis chinensis extract changed,such as depression and distortion,and most of them were dyed red,and the degree of morphological change and the proportion of dyed red were in direct proportion to the concen-tration of Coptis chinensis extract.The ATP content in MDR-Ab cells decreased gradually with the increase of the concentration of Coptis chinensis extract,and the ATP content outside the cells increased gradually with the increase of the concentration of Coptis chinensis extract.Conclusion Coptis chinensis extract has antibac-terial effect on MDR-Ab,and it can exert antibacterial effect by destroying MDR-Ab cell membrane.
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