Prevalence and analysis of norovirus in 16 clusters of vomiting and diarrhea outbreaks in Putuo District of Shanghai from 2018 to 2019
Objective To understand the genotyping and epidemiological characteristics of norovirus in 16 clusters of vomiting and diarrhea in Putuo District of Shanghai,and to provide technical support and data reference for the treatment and prevention of clusters of vomiting and diarrhea caused by norovirus in the dis-trict and the whole city.Methods A total of 136 anal swab samples were collected from people involved in 16 clusters of vomiting and diarrhea outbreaks in Putuo District of Shanghai from 2018 to 2019.The nucleic acid of norovirus was detected by real-time fluorescence polymerase chain reaction.The polymerase-capsid protein link region was amplified by real-time fluorescence polymerase chain reaction and sequenced in the positive samples of typical cases.Genotyping and phylogenetic tree were constructed by bioinformatics tools.Results Among the 136 specimens,62(45.59%)were positive for norovirus GⅡ nucleic acid,and zero was positive for norovirus GI nucleic acid.42 noroviruses were successfully sequenced,and four genotypes were obtained by sequence align-ment analysis.Among the 16 clusters of vomiting and diarrhea outbreaks,nine were GⅡ.P16-GⅡ.2(56.25%),two were GⅡ.P7-GⅡ.6(12.50%),two were GⅡ.P12-GⅡ.3(12.50%),and three were GⅡ.Pe-GⅡ.4(18.75%).Conclusion Norovirus is an important pathogen causing clustered vomiting and di-arrhea in Putuo District of Shanghai from 2018 to 2019.It is mainly GⅡ group and has a variety of genotypes,among which GⅡ.P16-GⅡ.2 is the main epidemic strain.
NorovirusCluster vomiting and diarrhea outbreaksGenotypingPrevalence analysis
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