催化发夹组装反应用于检测生物标志物的研究进展
Study on the application of catalytic hairpin assembly reaction in the detection of biomarkers
翁琴 1李建军 2杨丰宁 3艾军宇 4余华5
作者信息
- 1. 成都中医药大学医学与生命科学学院,四川 成都 611137
- 2. 陆军军医大学第一附属医院肿瘤科,重庆 400038
- 3. 成都中医药大学临床医学院,四川 成都 611137
- 4. 成都中医药大学护理学院,四川 成都 611137
- 5. 成都中医药大学附属医院普外科,四川 成都 610072
- 折叠
摘要
催化发夹组装(CHA)是一种不依赖酶生物催化作用和温度循环的高效核酸扩增方法,具有生物相容性好、操作简单、反应条件温和等特点.通过将CHA与各种信号输出模式相结合,可实现对各种生物靶标(核酸、小分子、蛋白质、细胞)的高灵敏度检测,在诊断疾病方面显示出巨大的应用潜力.该文主要介绍了经典CHA、改良CHA,以及基于CHA策略与其他等温无酶扩增方法的几种级联反应,并对其在诊断疾病方面的应用进行了简单总结.还讨论了当前CHA策略在诊断疾病方面所面临的挑战和应用前景.
Abstract
Catalytic hairpin assembly(CHA)is an efficient nucleic acid amplification method that does not depend on enzyme biocatalysis and temperature cycling,and is characterized by good biocompatibility,sim-ple operation,and mild reaction conditions.By combining CHA with various signal output modes,highly sensi-tive detection of various biological targets(nucleic acids,small molecules,proteins,cells)can be realized,which shows great potential for application in disease diagnosis.Therefore,this paper focuses on classical CHA,modified CHA,and several cascade reactions based on the CHA strategy with other isothermal enzyme-free amplification methods,and briefly reviews their applications in disease diagnosis.In addition,the challen-ges and application prospects of current CHA strategies in disease diagnosis are discussed.
关键词
催化发夹组装/生物标志物/信号放大策略/生物传感器/综述Key words
Catalytic hairpin assembly/Biomarkers/Signal amplification strategies/Biosensor/Review引用本文复制引用
基金项目
四川省科学技术厅重点研发项目(2022YFS0201)
四川省中医药管理局面上项目(2023MS637)
出版年
2024
国家科技期刊平台
NSTL
万方数据