Study on the application of catalytic hairpin assembly reaction in the detection of biomarkers
Catalytic hairpin assembly(CHA)is an efficient nucleic acid amplification method that does not depend on enzyme biocatalysis and temperature cycling,and is characterized by good biocompatibility,sim-ple operation,and mild reaction conditions.By combining CHA with various signal output modes,highly sensi-tive detection of various biological targets(nucleic acids,small molecules,proteins,cells)can be realized,which shows great potential for application in disease diagnosis.Therefore,this paper focuses on classical CHA,modified CHA,and several cascade reactions based on the CHA strategy with other isothermal enzyme-free amplification methods,and briefly reviews their applications in disease diagnosis.In addition,the challen-ges and application prospects of current CHA strategies in disease diagnosis are discussed.
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