Study on the mechanism of hepatocyte pyroptosis induced by LncRNA-NEAT1 regulation of the miR-129-5p/HMGB1 axis in acute liver injury
Objective To investigate the mechanism of long non-coding RNA nuclear-enriched abundant transcript 1(LncRNA-NEAT1)in inducing hepatocyte pyroptosis in acute liver injury through the regulation of the miR-129-5p/high mobility group box 1(HMGB1)axis.Methods Human hepatocyte line L02 was used to establish a model of hepatocyte injury induced by carbon tetrachloride(CCl4).Alanine aminotransferase(ALT)and aspartate aminotransferase(AST)levels were measured to assess the degree of hepatocyte injury.Cell proliferation activity was detected using the CCK-8 method,and interleukin(IL)-1β and IL-18 levels were measured to evaluate the extent of cell pyroptosis.Real-time quantitative polymerase chain reaction(RT-PCR)was used to detect the expression levels of LncRNA-NEAT1 and miR-129-5p mRNA.After interfering with NEAT1 expression and treating cells with CCl4,ALT,AST,IL-1β,and IL-18 levels were measured in the control and treatment groups,and RT-PCR was used to detect the expression levels of LncRNA-NEAT1 and miR-129-5p mRNA.After interfering with NEAT1 expression,miR-129-5p mimics were transfected,and cells were treated with CCl4.ALT,AST,IL-1β,and IL-18 levels were measured in the control and treatment groups,and RT-PCR was used to detect the expression levels of LncRNA-NEAT1 and miR-129-5p mRNA.Dual-luciferase reporter gene assays were performed to verify the relationship between NEAT1 and miR-129-5p,and miR-129-5p and HMGB1,respectively.Results After CCl4 treatment,ALT and AST levels in hepato-cytes increased significantly,IL-1β and IL-18 expression levels increased,cell activity decreased,NEAT1 ex-pression increased,and miR-129-5p expression was inhibited,with statistically significant differences(P＜0.05).After NEAT1 interference,ALT,AST,IL-1β,and IL-18 levels in hepatocytes decreased,and miR-129-5p expression increased significantly,with statistically significant differences(P＜0.05).After NEAT1 inter-ference and miR-129-5p mimic transfection,ALT,AST,IL-1β,and IL-18 levels in hepatocytes were inhibited,NEAT1 expression was reduced,and miR-129-5p expression was increased,with statistically significant differ-ences(P＜0.05).NEAT1 targetedly regulated miR-129-5p expression,and miR-129-5p targeted HMGB1.Conclusion Interfering with NEAT1 may inhibit hepatocyte pyroptosis in CCl4-induced acute liver injury by targeting the miR-129-5p/HMGB1 axis.
LncRNA-NEAT1miR-129-5pHigh mobility group box 1HepatocytePyropto-sisAcute liver injury
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