Effects of bufrudin on lipid metabolism and vascular plaque in ApoE-/-mice atherosclerosis
Objective To investigate the effect of bufrudin on lipid metabolism and vascular plaques in ApoE-/-mice with atherosclerosis.Methods A total of 24 male ApoE-/-mice fed with high-fat diet were randomly divided into the model group,the high-dose and the low-dose groups of bufrudin,and the simvastatin positive group,with six mice in each group.Another six C57BL/6J mice were taken as the blank control group and fed with normal diet.After continuous administration for eight weeks,the blood lipid level was detected after the last administration,and the pathological changes of aortic vessels were observed by HE staining and Masson staining.Immunohistochemical staining was used to observe the effects of bufrudin on proliferating cell nuclear antigen(PCNA)and caspase-3 protein in aortic atherosclerotic plaques of mice.Results Com-pared with the blank control group,the levels of serum total cholesterol(TC),triglyceride(TG)and low den-sity lipoprotein cholesterol(LDL-C)in the model group were significantly increased,and the level of high den-sity lipoprotein cholesterol(HDL-C)was significantly decreased,and differences were statistically significant(P＜0.01).Compared with the model group,the levels of serum TC,TG and LDL-C in the high-dose and low-dose groups of bufrudin were significantly decreased,and the level of HDL-C was significantly increased,the differences were statistically significant(P＜0.01).Masson staining showed that compared with the model group,the collagen content in the vascular plaque of mice in the high and low dose groups of bufrudin was sig-nificantly increased,and the difference was statistically significant(P＜0.01).The results of immunohisto-chemical staining showed that compared with the blank control group,the expression of PCNA protein in the aortic atherosclerotic plaque of the model group was significantly down-regulated,and the expression of caspase-3 protein was significantly up-regulated,the differences were statistically significant(P＜0.01).Com-pared with the model group,the expression of PCNA protein in the aortic atherosclerotic plaque of the mice in the bufrudin group was significantly up-regulated,and the expression of caspase-3 protein in the bufrudin high-dose group was significantly down-regulated,the differences were statistically significant(P＜0.05).Conclusion Bufrudin can inhibit the development of atherosclerosis by reducing serum lipid levels in ApoE mice,delaying smooth muscle cell apoptosis,and stabilizing vascular plaques.
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