Objective To verify the performance of the flow microsphere capture chip technology in the detection of various cytokines in plasma.Methods According to the standard documents of the health indus-try of the People's Republic of China and the American Society of Clinical and Laboratory Standards,the six cytokines of interleukin(IL)-1β,IL-2R,IL-6,IL-8,IL-10 and tumor necrosis factor α(TNF-α)in plasma were detected by flow microsphere capture chip technology,and the precision,accuracy,linear range,reference inter-val,minimum detection limit and anti-interference ability were evaluated.Results The intra-batch precision coefficient of variation(CV)of the six cytokines was 1.50％-8.83％,and the inter-batch precision(CV)was 2.48％-6.75％.The relative deviation of accuracy was ≤±15％.The concentration range of IL-1β,IL-6,IL-8,IL-10 and TNF-α was 2.5-5 000.0 pg/mL,and the concentration range of IL-2R was 5-7 500 U/mL.The linear range was good.The verification results of biological reference interval showed that 90％of the re-sults were within the range of biological reference interval provided by the manufacturer.The minimum detec-tion limits of IL-1β,IL-6,IL-8,IL-10 and TNF-α were ≤ 2.5 pg/mL,and the minimum detection limit of L-2R was ≤ 5 U/mL.The interfering substances(triglyceride 10 mmol/L,hemoglobin 5 mg/L and bilirubin 258 μmol/L)had no effect on the test results of the kit.Conclusion The method of detecting plasma cyto-kines by flow cytometric bead capture chip technology on mindray Bricyte E6 flow cytometry has good per-formance,which can provide accurate and reliable quantitative detection results of six cytokines for clinical practice.
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