Methodological performance verification of flow microsphere capture chip technology for detecting plasma cytokines
Objective To verify the performance of the flow microsphere capture chip technology in the detection of various cytokines in plasma.Methods According to the standard documents of the health indus-try of the People's Republic of China and the American Society of Clinical and Laboratory Standards,the six cytokines of interleukin(IL)-1β,IL-2R,IL-6,IL-8,IL-10 and tumor necrosis factor α(TNF-α)in plasma were detected by flow microsphere capture chip technology,and the precision,accuracy,linear range,reference inter-val,minimum detection limit and anti-interference ability were evaluated.Results The intra-batch precision coefficient of variation(CV)of the six cytokines was 1.50％-8.83％,and the inter-batch precision(CV)was 2.48％-6.75％.The relative deviation of accuracy was ≤±15％.The concentration range of IL-1β,IL-6,IL-8,IL-10 and TNF-α was 2.5-5 000.0 pg/mL,and the concentration range of IL-2R was 5-7 500 U/mL.The linear range was good.The verification results of biological reference interval showed that 90％of the re-sults were within the range of biological reference interval provided by the manufacturer.The minimum detec-tion limits of IL-1β,IL-6,IL-8,IL-10 and TNF-α were ≤ 2.5 pg/mL,and the minimum detection limit of L-2R was ≤ 5 U/mL.The interfering substances(triglyceride 10 mmol/L,hemoglobin 5 mg/L and bilirubin 258 μmol/L)had no effect on the test results of the kit.Conclusion The method of detecting plasma cyto-kines by flow cytometric bead capture chip technology on mindray Bricyte E6 flow cytometry has good per-formance,which can provide accurate and reliable quantitative detection results of six cytokines for clinical practice.
万方数据
维普
