Comparison of the fast paraffin technology of ultrasound and microwave principles
Objective To compare the application effects of rapid paraffin techniques based on two dif-ferent principles:ultrasound and microwave.Methods Rapid paraffin samples processed by ultrasonication and microwave methods from January to December 2022 at the Department of Pathology,the First Affiliated Hospital of Soochow University were collected.The application effects of these two techniques were compared by analyzing the excellent rate of Hematoxylin and Eosin(HE)staining,immunohistochemical staining,fluo-rescence in situ hybridization(FISH)outcomes,processing time,and reagent environmental friendliness.Re-sults A total of 1 989 rapid paraffin samples were processed using ultrasonication,and 1 540 samples were processed using the microwave method.Both techniques were extensively applied to small tissue biopsies from various parts of the body,with an excellent HE slide quality rate reaching 99％.There was no statistically sig-nificant difference in HE staining scores between the two techniques(P＞0.05).Three instances of slide de-tachment were observed in samples processed by ultrasonication(detachment rate of 0.15％),compared to eight in the microwave-processed samples(detachment rate of 0.53％);however,the difference was not statis-tically significant(P＞0.05).Both techniques provided satisfactory results in immunohistochemical staining;the FISH detection outcomes of rapid paraffin slices processed by both techniques were satisfactory and did not affect result interpretation.The processing time for ultrasonication rapid paraffin technique was 69(63,76)minutes,whereas it was 123(119,126)minutes for the microwave technique,showing a statistically significant difference(P＜0.05).Conclusion Both rapid paraffin techniques produced an excellent slide quality rate of o-ver 99％,and consistent results in HE staining,immunohistochemistry,and FISH detection.However,consid-ering the overall detachment rate and tissue processing time,the ultrasonication-based rapid paraffin technique is more deserving of clinical promotion.
万方数据
维普
