Exploring the hypotensive mechanisms of action of tianma based on network pharmacology
Objective To explore the potential mechanisms of asparagus in lowering blood pressure through cyberpharmacology.Methods The targets of essential hypertension were obtained from Disgenet and Genecards databases,and the targets of tianma components were obtained from the BATMAN-TCM data-base;candidate"drug-disease"targets were identified by screening the intersection of disease targets and drug component targets.The top 10 signaling pathways were screened by using ExpressAnalyst for gene ontology enrichment analysis and WebGestalt for Kyoto Gene and Genome Encyclopedia enrichment analysis.Protein interaction networks were constructed using the STRING database and the top 10 key targets were screened by Cytohubba.Cytoscape software was used to construct"drug-component-target-disease"and"component-target-pathway"network diagrams.Results A total of 207 disease targets of essential hypertension were screened,and 14 active components of Tianma were screened,of which 8 components could regulate 27 disease targets of essential hypertension.These targets were mainly enriched in circadian rhythms,vasoconstriction,cellular calcium homeostasis,rhythmicity and other biological processes;the molecular functions involved in-cluded nuclear receptor activity,cytokine activity and hormone activity,and the targets were mainly distribu-ted in the extracellular cytoplasmic region.Signaling pathways include longevity signaling pathway,Chagas disease signaling pathway,neuroactive ligand-receptor interaction signaling pathway,interleukin-1 7 inflamma-tory signaling pathway and so on.The main active ingredients of Tianma,such as 4-hydroxybenzylamine,nor-bornanine,sucrose,m-hydroxybenzoic acid,γ-sitosterol,affect the first 10 key signaling pathways through the modulation of 8 key targets of TNF,PPARG,INS,ADIPOQ,NFKB1,MMP9,SERPINE1,and IFNG,thus modulating essential hypertension.Conclusion The antihypertensive mechanism of tianmu,a traditional Chi-nese medicine,involves a network of multi-components,multi-targets and multi-pathways regulation.
万方数据
维普
