Transcriptome analysis of high-fat diet affecting intestinal immune function in mice
Objective To investigate the effects of high-fat feeding on intestinal immune function and related gene expression in mice,and to explore the potential mechanisms by which a high-fat diet leads to in-testinal inflammation and affects intestinal barrier function.Methods In June 2019,A total of 24 C57BL/6J male mice were divided into a normal diet group(CON group)and a high-fat diet group(HFD group),with 12 mice in each group,using the random grouping methed,divided into 4 cages with 3 mice in each cage,and fed for 18 weeks respectively.Enzyme-linked immunosorbent assay(ELISA)kits were used to detect bio-chemical indexes such as blood glucose and blood lipids.Total RNA of mouse cecum tissue was extracted by Trizol reagent method,cDNA was obtained by reverse transcription and libraries were constructed for RNA transcriptome sequencing,and differentially expressed genes were subjected to functional analysis and pathway enrichment analysis by using Gene Ontology,Kyoto Genome and Genome Encyclopedia databases.Results The body weight[(36.96±1.31)g],and GLU[(6.65±0.41)mmol/L],LDL cholesterol[(0.97±0.07)mmol/L],total cholesterol[(4.08±0.15)mmol/L],and triacylglycerol[(0.64±0.03)mmol/L]of the mice in the HFD group at week 18 levels were significantly higher than those in the CON group[(31.81±0.53)g,(4.53±0.33),(0.60±0.04),(2.79±0.13),and(0.51±0.02)mmol/L,respectively],and the differences were statistically significant(t=3.66,4.00,4.46,6.34,3.52,P=0.001 4,0.000 6,0.000 2,＜0.000 1,0.002 0).The differentially expressed genes in cecum tissues were mainly enriched to several biologi-cal processes such as immunity and cell death,in which the major inflammatory factors such as cxcr4,ccr7,cx-cl12,ccl28,cxc113,and ccl21a were significantly up-regulated,and the high-fat diet markedly reduced the im-portant genes for the intestinal barrier function,the cndl4 expression,which may lead to impaired barrier func-tion.Conclusion A high-fat diet can significantly affect intestinal immune function and may induce impaired intestinal barrier function,which is a potential causative factor for intestinal inflammation and inflammation-related diseases.
万方数据
维普
