Mur blood group antigen gene testing and antibody screening among voluntary blood donors in Yueyang,Hunan Province
Objective To investigate the distribution frequency of Mur blood group antigens and the in-cidence of anti-Mur among voluntary blood donors in Yueyang area of Hunan Province,so as to provide guar-antee for the safety of clinical blood transfusion.Methods A total of 4 660 voluntary blood donors in this sta-tion were selected from January to June 2023.Among them,437 samples were tested for Mur antigen by poly-merase chain reaction-sequence specific primers,and 4 223 samples were tested for anti-Mur by serological method.The samples with agglutination reaction were identified by red blood cell antibody and the titer was determined.Mur antigen gene sequencing was performed in two cases.Results The distribution frequency of Mur antigen was 3.66％(16/437),the incidence of anti-Mur was 0.17％(7/4 223)in 4 660 blood donors,and the antibody type was immunoglobulin M type.The sequencing results of the Mur antigen gene were consist-ent with Mur antigen of the International Society of Blood Transfusion.Conclusion The distribution of Mur antigen and anti-Mur in blood donors in Yueyang,Hunan Province has a certain frequency.In order to ensure the safety of clinical blood transfusion and improve the experimental diagnosis of hemolytic disease of new-born,Mur antigen red blood cells should be added,which can be detected by molecular biological diagnostic re-agents.In order to ensure the safety of clinical blood transfusion,rare blood group library should be estab-lished when necessary.
万方数据
维普
