首页|阳离子脂质纳米微泡超声造影剂的制备及特性研究

阳离子脂质纳米微泡超声造影剂的制备及特性研究

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目的 通过自制阳离子脂质纳米微泡超声造影剂,探讨一种更稳定、高效的基因载体工具,并比较自制阳离子脂质纳米微泡和普通脂质微泡的制备效果.方法 使用二硬脂酰基磷脂酰胆碱(DSPC)/二硬脂酰基磷脂酰乙醇胺-聚乙二醇(DSPE-PEG2000)/DC-胆固醇(DC-Chol)按摩比为9∶0.5∶1制备阳离子脂质纳米微泡,以单纯微泡——核心纳米微泡为对照,测定各组微泡的一般理化性质指标及其稳定性,包括粒径、表面电位、分散性指数(PDI)等,利用倒置显微镜、电镜观察各组微泡形态,并通过体外琼脂糖模型观察其体外造影效果.结果 构建的阳离子脂质纳米微泡平均粒径为(564.35±25.93)nm,平均表面电荷为(17.6±2.1)mV,PDI为0.30±0.16,浓度为(4.27±0.31)×108;核心纳米微泡平均粒径为(582.55±16.30)nm,平均表面电荷为(-7.9±1.6)mV,PDI为0.37±0.15,浓度为(4.24±0.30)× 108;2组表面电荷比较,差异有统计学意义(P<0.05),在连续测定2 h内均保持了较好的稳定性,体外造影效果良好.结论 自制的阳离子脂质纳米微泡具有较高的正电荷,同时保持较好的稳定性及体外造影效果,该方法有望推广应用并为搭载配体提供参考,成为高效的新型造影剂.
Preparation and characterization of cationic lipid nanobubbles as ultrasound contrast agents
Objective To explore a more stable and efficient gene carrier tool by preparing cationic lipid nanobubbles as ultrasound contrast agents and to compare the preparation effects of homemade cationic lipid nanobubbles with those of ordinary lipid microbubbles.Methods Cationic lipid nanobubbles were prepared u-sing distearoylphosphatidylcholine(DSPC)/distearoylphosphatidylethanolamine-polyethylene glycol(DSPE-PEG2000)/DC-cholesterol(DC-Chol)at a molar ratio of 9:0.5:1.Simple microbubbles,namely,core nanobubbles,were used as controls.The general physicochemical properties and stability of the nanobubbles in each group were measured,including particle size,surface potential,polydispersity index(PDI),etc.The mor-phology of the nanobubbles in each group was observed using an inverted microscope and an electron micro-scope,and their contrast effects in vitro were observed using an agarose gel model.Results The prepared cat-ionic lipid nanobubbles had an average particle size of(564.35±25.93)nm,an average surface charge of(17.6±2.1)mV,a PDI of 0.30±0.16,and a concentration of(4.27±0.31)×108.The core nanobubbles had an average particle size of(582.55±16.30)nm,an average surface charge of(-7.9±1.6)mV,a PDI of 0.37±0.15,and a concentration of(4.24±0.30)×108.There was a statistically significant difference in surface charge between the two groups(P<0.05).Both groups maintained good stability within 2 hours of continu-ous measurement and showed good contrast effects in vitro.Conclusion The homemade cationic lipid nanobubbles have high positive charges while maintaining good stability and contrast effects in vitro.This method is expected to be promoted and applied and provides a reference for ligand loading,potentially becom-ing an efficient and novel contrast agent.

Ultrasound microbubblesContrast agentsCationicNanoscalePreparationCharacterization study

袁晨、吴治胜、穆玉明

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新疆医科大学第一附属医院心脏超声诊断科,新疆乌鲁木齐 830011

新疆超声医学重点实验室,新疆乌鲁木齐 830011

超声微泡 造影剂 阳离子 纳米 制备 特性研究

2024

现代医药卫生
重庆市卫生信息中心

现代医药卫生

影响因子:0.758
ISSN:1009-5519
年,卷(期):2024.40(24)
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