Study on the effect of dendrobium candidum polysaccharides on endothelial progenitor cells in atherosclerotic rats
Objective To study the effect of Dendrobium candidum polysaccharides on endothelial pro-genitor cells(EPCs)in atherosclerotic(AS)rats.Methods After one week of adaptive feeding,rats were ran-domly divided into six groups:NG group(normal group),AS group,Res group(resveratrol group),DPH group(high-dose Dendrobium candidum polysaccharides group),DPM group(medium-dose Dendrobium can-didum polysaccharides group),and DPL group(low-dose Dendrobium candidum polysaccharides group).Ex-cept for the NG group,rats in the other groups received a single intraperitoneal injection of 7×105 U/kg of vi-tamin D3 and were fed with high-fat feed for 9 weeks to establish the AS model;rats in the NG group were fed with normal feed and received an intraperitoneal injection of the same volume of saline.After successful mod-eling,rats in the DPH,DPM,and DPL groups were gavaged with Dendrobium candidum polysaccharides at do-ses of 8,4,and 2 g/mL respectively,with a dosing volume of 40 mL/kg.Rats in the Res group were gavaged with 100 mg/kg of resveratrol daily,while rats in the NG and AS groups were gavaged with 100 mg/kg of sa-line daily.Nine weeks later,the rats were euthanized,and bone marrow was collected from the femurs to iso-late mononuclear cells,which were then cultured and induced to differentiate into EPCs.The proliferation,ad-hesion,and migration abilities of EPCs were measured.Results The proliferation,adhesion,and migration a-bilities of EPCs in the AS group were significantly lower than those in the NG group,while the proliferation,adhesion,and migration abilities of EPCs in the DPH,DPM,DPL,NG,and Res groups were higher than those in the AS group,with statistically significant differences(P＜0.05).Conclusion Dendrobium candidum poly-saccharides promote the proliferation,adhesion,and migration abilities of EPCs in AS rats,indicating that Dendrobium candidum polysaccharides have a potential therapeutic effect on AS.
Dendrobium candidumPolysaccharidesAtherosclerosisEndothelial progenitor cellsProliferationMigrationAdhesionMechanism of action
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维普
