Qi Di Tang Shen decoction mediates high glucose-stimulated glomerular endothelial cells'pyroptosis via regulating NLRP3/Caspase1/GSDMD pathway
Objective It is to investigate the effect of Qi Di Tang Shen decoction on high glucose-stimulated glomerular endothelial cells(mGECs)via Nod-like receptor protein 3/cystein-asparate protease-1/gasdermin D(NLRP3/Caspase1/GSDMD)pathway.Methods The mGECs were divided into three groups,named as normal group,high glucose group and Qi Di Tang Shen decoction group,which were respectively cultured in complete medium,30 mmol/L high-glucose medi-um,30 mmol/L high-glucose medium +100 μg/mL Qi Di Tang Shen decoction for 48 h.The expression of NLRP3 in cells and cytoskeleton were observed by cellular immunofluorescence staining,the expressions of NLRP3,Caspase1,apoptosis-associated spot-like protein(ASC),GSDMD,interleukin-1β(IL-1β)and interleukin-18(IL-18)in mGECs were detec-ted by cellular immunofluorescence staining.Results Compared with the normal group,the relative expressions of NLRP3,Caspase1,GSDMD,ASC,IL-18 and IL-1β in mGECs of the high glucose group were significantly increased(all P＜0.05),the fluorescent expression of NLRP3 was enhanced,and the cytoskeleton damage was obvious.Compared with the high glucose group,the relative expressions of NLRP3,Caspase1,GSDMD,ASC,IL-18 and IL-1β in Qi Di Tang Shen decoction group were significantly down-regulated(all P＜0.05),and the fluorescent expression of NLRP3 was decreased and the cytoskeletal damage was improved.Conclusion Qi Di Tang Shen decoction can improve the high-glucose induced injury of glomerular endothelial cells by inhibiting the activation of NLRP3/Caspase1/GSDMD pathway.
diabetic kidney diseaseglomerular endothelial cellsQi Di Tang Shen decoctionpyroptosisNod-like receptor protein 3cystein-asparate protease-1gasdermin D
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维普
