Effect difference of electroacupuncture at different specific acupoints on chronic colitis in rats
Objective It is to observe the effect different and mechanism of electroacupuncture at Tianshu,Dachang-shu,Zusanli and Shangjuxu on inflammation of colon.Methods Sixty SD rats were randomly divided into normal group,model group,Tianshu group,Dachangshu group,Zusanli group,Shangjuxu group after feed for one week,with 10 rats in each group.The rats of all groups except for the normal group were prepared by immune compound sensitization method to establish models of chronic colitis.Each acupoint treatment group was treated with electroacupuncture at corresponding acu-point after modeling,with the parameters of 2 Hz/50 Hz,current intensity of 2 mA,20 min/time,once daily,continuously treated for 10 days;the rats of the normal group and model group were only fixed with the same method at the same time.The Disease Activity Index(DAI)score of the rats was calculated according to their body weight,stool condition,and stool blood,and the Colonic Mucosal Damage Index(CMDI)score was calculated according to their colonic mucosal injury;the pathological morphology of colonic tissues was observed by HE staining;the serum levels of interleukin-10(IL-10)and tumor necrosis factor-alpha(TNF-α)were detected by ELISA;the protein expressions of matrix metalloproteinase-1(MMP-1)and matrix metalloproteinase-2(MMP-2)in colonic tissues were detected by Western blotting.Results Com-pared with the normal group,the DAI scores,CMDI scores,the serum level of TNF-α and the protein relative expressions of MMP-1 and MMP-2 in colonic mucosa of rats in the model group were significantly increased(all P＜0.05),with infil-tration of a large number of inflammatory cells in colonic tissue.Compared with the model group,the general condition and colon tissue injury of rats in each acupoint treatment group were significantly improved,the DAI scores,CMDI scores,ser-um level of TNF-α and the protein relative expressions of MMP-1 and MMP-2 protein in colon mucosa were significantly de-creased(all P＜0.05),the serum level of IL-10 was significantly increased(P＜0.05);the improvements of the above indexes in Shangjuxu group were significantly better than those in Tianshu group,Dachangshu group and Zusanli group(all P＜0.05).Conclusion Electroacupuncture at Tianshu,Dachangshu,Zusanli and Shangjuxucan can repair colonic muco-sal injury and alleviate intestinal inflammation in rats with chronic colitis,the intervention effect of electroacupuncture at Shangjuxu point on chronic colitis may be relatively specific.
万方数据
维普
